sodium palmitate is the esther or salt of palmitic acid is not soluble in water and polar solutionç. palmitic acid is to work in the fattyacid metabolizim well  palmitate is more a endproduct,  in the cell  Acyl-Hydrolase transform palmitic acid to palmitate. i think palmitic acid is good as palmitate for İR modeling in cell culture  because you invoke the metabolic action of the cell thats can ending with IR, like as in the obez human population with DM2 and IR.

The reason why some people refer to palmitic acid solution as sodium palmitate is that they add NaOH when preparing PA solution. We do it in our lab. I find it more reliable way to prepare PA solution than ethanol. I used to use ethanol in my previous lab, but now use only NaOH. I can give a protocol we use in our lab.

I agree with Evgeniy. We found using palmitic acid (Dissolved in NaOH) more reliable and physiological (as free fatty acids are transported after being attached to albumin in blood). 

Hi Evgeniy,

Could you please send the protocol that you used in your lab?

Evgeniy, I would also appreciate the protocol for the prep of Sodium palmitate if possible. Thanks very much.

Evgeniy Panzhinskiy please send the protocol that you used in your lab!!

Evgeniy Panzhinskiy

I would  also appreciate the protocol regarding to preparation os sodium palmitate if you don't mind. thanking you in advance.

Hi Evgeniy, 

May I please also have a copy of the protocol? My email is:

grace(dot)yeung(at)mater(dot)uq(dot)edu(dot)au

Thank-you!

Grace

Palmitic Acid powder from sigma p0500 I believe. dilute to 100 mM in 0.1M NaOH and heat at 70C for 30 mins then briefly vortex and incubate for another 15 mins at 70C to completely disolve. Add heated 100 mM PA in NaOH to warm (50 - 55C) 1 - 10% fatty acid free BSA to get to desired concentration and vortex for 10 seconds. Place ALB-PA at 50 - 55C for another 15 mins then let cool, filter and use. Can be stored for up to a month at -20C.

I am trying to make a palmitic oil solution to immerse a material in for a few days, maybe a couple weeks, at room temperature and at 37C. Can I use the same protocol as Dwayne Carter mentioned?

Thanks,

Maria

Evgeniy Panzhinskiy

Hi Evgeniy,

May I please also have a copy of the protocol that you used in your lab for sodium palmitate?

My email is:

[email protected]

Thanks,

Suzan

Evgeniy Panzhinskiy

Hello Evgeniy,

May I please also have a copy of the protocol that you used in your lab for preparation of Sodium Palmitate?

To this emails:

[email protected]

[email protected]

Thanks,

Inma

@Evgeniy Panzhinskiy HI, Evgeniy, how do you do? I am sorry it is an old topic, though I am trying to make a PA solution to add in the cell these days. Can you share the protocal for PA solution? By the way , I bought sodium palmitate and sodium oleate from sigma, not palmitic acid. Do you know how to dissolve the sodium palmitate and sodium oleate? my email: [email protected] thank you very much.

Hello Mr Panzhinskyi, I’m beginning my master thesis this month and I need to complex BSA with stearic acid. In our lab we don’t have sodium stearate so I know that the protocol is different, that’s why I’m asking you if you would like to send me the protocol you use in your lab

Hi can I also get the protocol of the same

my email is [email protected]

May I also get a copy of the protocol?

My email is [email protected]

I want to induce hyperlipidemia in HepG2 cells using pa (sigma) and bsa faf.

can you share the protocol here with me ?