Does anyone have experience regarding the difference in metastatic potential of B16-F10 and B16-F1 cells? How much longer would B16-F1 cells need to cause a substantial number of metastasis in the mouse lung when injected i.v.?
I found quite different statements in literature. Some showing almost no metastasis, some showing a huge number of metastasis after 2 weeks.
Thanks in advance!
Dear Carsten,
I am afraid you are making a minor mistake in regard the concept of metastasis and colonization. When you intravenously inject the B16 cells you are bypassing important steps of the metastatic process, e.g. invasion and intravasation. If you are studying extravasation and colonization, the model of Lung colonization is a good option. The time of metastasis occurrence totally depends on the number of cells you inject. I use to inject 3x10e4 to 10e6 of B16-F10 cells intravenously, with good results at D+14 to D+21. By the other side, if you want to study the whole metastatic process, I recommend you trying to model a primary tumor (e.g. subcutaneous melanoma). I noticed good number of spontaneous metastasis (lungs, brain, liver) at D+42. Another option is assessing spontaneous metastasis through survival. For example, you inject B16-F10 subcutaneously and wait until D+14 to D+18. Then you surgically remove the primary tumor and observe the survival of your different groups. Unless the animal develop a local relapse, you can state that the mortality is due to spontaneous mets. About the other part of your question, the literature claims that B16-F10 is a more metastatic strain of B16, so I would recommend you to test them, in opposite of B16-F1.
Dear Carsten,
I am afraid you are making a minor mistake in regard the concept of metastasis and colonization. When you intravenously inject the B16 cells you are bypassing important steps of the metastatic process, e.g. invasion and intravasation. If you are studying extravasation and colonization, the model of Lung colonization is a good option. The time of metastasis occurrence totally depends on the number of cells you inject. I use to inject 3x10e4 to 10e6 of B16-F10 cells intravenously, with good results at D+14 to D+21. By the other side, if you want to study the whole metastatic process, I recommend you trying to model a primary tumor (e.g. subcutaneous melanoma). I noticed good number of spontaneous metastasis (lungs, brain, liver) at D+42. Another option is assessing spontaneous metastasis through survival. For example, you inject B16-F10 subcutaneously and wait until D+14 to D+18. Then you surgically remove the primary tumor and observe the survival of your different groups. Unless the animal develop a local relapse, you can state that the mortality is due to spontaneous mets. About the other part of your question, the literature claims that B16-F10 is a more metastatic strain of B16, so I would recommend you to test them, in opposite of B16-F1.
I agree with Jose comments. You could also try B16BL6 cells. It has higher metastatic potential than B16F10.
Good luck!
It was reported by Prof Fidler and his colleagues about the beginning of 1980s. They injested B16 tumor cells into footpad of mice and collected pulmonary tissues to regrow tumor cells in vitro--call B16-F2. The B16-F2 tumor again injected into footpad normal mice and after amount of time, pulmonary tissues were culture and tumor cells was called B16-F3,----. Now many commercial B16-F10 have been serially transplanted in vivo or in vitro, their metastatic abilities are complemised by these transplantation.
Here are the references relating to the various B16 variants.
The B16F0 model (usually named B16) is not metastatic.
The B16F10 variant is PSEUDOMETASTATIC: I fully agree with the crystal-clear response of Jose Mauricio.
You can indeed inject B16F10 melanoma cells into the tail vein to obtain lung COLONIZATION (or lung PSEUDOMETASTASES; see Mathieu et al., 2007) or in the carotid to have brain COLONIZATION (brain PSEUDOMETASTASES; see another attached article).
Dear Vijayendra,
I am actually interested by the B16BL6 model that was never used in my research unit. Would you be so kind in sending me one or two "good" articles demonstrating the actual metastasizing properties of this model, as opposed to the "pseudometastatic" ones of the B16F10 model.
Thank you very much.
Best regards
Robert
Hi Robert! Sorry for the late response. You can see my paper... I have also used B16BL6 cell line for lung and lymph node metastasis.
http://www.ncbi.nlm.nih.gov/pubmed/24811731
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