SPiIKE OF SAMPLE: addition of known concentration of standard drug to the pre quantified sample.
RECOVERY CHECK: how much amount of drug recovered by proposed analytical method after spiked the sample with std drug.
These two concepts very important in accuracy studies in method validation.
Recovery check= concn of sample+ spiked std drug cocen.
% RECOVERY= total amount recovered by proposed method/ actual total amount X 100.
SPiIKE OF SAMPLE: addition of known concentration of standard drug to the pre quantified sample.
RECOVERY CHECK: how much amount of drug recovered by proposed analytical method after spiked the sample with std drug.
These two concepts very important in accuracy studies in method validation.
Recovery check= concn of sample+ spiked std drug cocen.
% RECOVERY= total amount recovered by proposed method/ actual total amount X 100.
It is essentially the same term. In order to check recovery you add (spike) a known concentration of analyte into a sample that you have quantified. There are other terms; for example, FDA calls it "sample fortification". Different terminologies, same end result.
In addition to the above discussions:
The method of ‘standard additions’ is a fundamental method for analyte determination because it minimizes errors produced by composition and physical properties of sample solution (without any separation of analyte of interest from the accompanying matrix. Truly, it is a simple means of verifying analytical values obtained by analytical working curves without the need for using another analytical instrumental method as a reference method. In fact, it is a self-calibration or auto-standardization method and is well documented in the literature.
Article Trends in the Methods of Measurement in Analytical Chemistry
what is the difference between % of spiked level and amount of drug added to calculate recovery %. ?
as of all above have been suggested,
recovery is just the analytical quantity of result obtain after some quantity of added amount with in the previous amount { [(antenatal sample concentration + concentration of added quantity)- concentration of analyt in the sample / analyte concentration in sample] }
the % recovery will be = { [(antenatal sample concentration + concentration of added quantity)- concentration of analyt in the sample / analyte concentration in sample] } x 100
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