I need to prepare Oxidation fermentation medium, the formula of preparation are
- Peptone.........................................................2.0g
- Sodium choloride..........................................5.0g
- dI- Potassium Hydrogen phosphate (K2HPO4).....0.3g
- Agar...........................................................................2.5g
- Bromothymol blue, 10g/l..........................................3ml
- Distiled water.............................................................1litre.
The question is i have Potassium dIhydrogen phosphate (KH2PO4 ) istead of dI- Potassium Hydrogen phosphate (K2HPO4).
So can i use KH2PO4 altarnatively for K2HPO4?
Your answer is very important to me.
Regards
Shafie Abdulkadir Hassan
Dear Shafie,
Both mono-potassium and di-potassium phosphates can supply phosphate to a culture media; this component is essential for microorganisms growth. But selection of one or the other phosphate salt is made function on the pH that is required to be obtained so as get a stabile buffered solution and optimal growth conditions. In order to get a neutral or close to neutral pH both salts should be used in various ratios. If use di-potassium phosphate salt (dibasic salt) as it is required in your formula, the fermentation media will be more alkaline (pH 7.4-7.6). If use mono-potassium phosphate, your prepared media will be more acid (pH
This link may be helpful to prepare phosphate buffers of different concentrations.
https://www.aatbio.com/resources/buffer-preparations-and-recipes/phosphate-buffer-ph-5-8-to-7-4
Can you then adjust pH to the required one by using NaOH?? @Carmen Mateescu
Can you then adjust pH to the required one by using NaOH?? @Carmen Mateescu
@Carmen mateescu Can you then adjust pH to the required one by using NaOH??
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