Why Negative peak eluted in HPLC analysis?

I'facing problem while doing HPLC analysis due to negative peak was eluted which was corresponding to the principle peak RT. I tried to remove, with different solvents, but no use. i want to know...

26 November 2020 1,960 5 View

Is there any list available of GRAS (generally recognized as safe) microorganisms with proper references?

Is there any list available of GRAS (generally recognized as safe) microorganisms with proper references?

02 March 2020 1,011 2 View

For an HPLC System, spectrum bandwidth mentioned as 8 nm. How to calculate this spectrum bandwidth?

Most of the HPLC Brochures/Specifications sheet mentions 'spectrum bandwidth' as 8 nm. But, how to calculate this one. Please suggest.

12 December 2019 4,770 3 View

Is there any Alternative for Sodium Naphthalene Sulfonate?

I am looking for alternate to SNF's (Dispersing Agent). The present one is in yellow colour and I am looking for the similar type of Dispersing agent in white colour.

29 August 2019 7,921 1 View

Which stain is used to see the deposition of oil or oil glands in anatomy section of leaf(transverse section) in cymbopogon?

Cymbopogon is an aromatic grass which produces essential oils as a secondary metabolite so to see the deposition of oil in leaves transverse section of intra and inter species can you suggest me...

28 August 2019 9,357 2 View

What is the difference between lithium ion and lithium polymer battery?

Lithium ion and lithium polymer battery charging and discharging profile was found to be same. Then what is the major difference between both battery?

07 July 2019 9,301 4 View

Homing Receptors for tumor infiltrating T cells?

I am researching how different culture and expansion conditions contribute to a T cell's infiltration of a solid tumor. Was wondering what the best surface / intracellular receptors or ligands are...

26 June 2019 5,072 0 View

What are my THP-1 cells contaminated with?

Hello everyone, I recently thawed THP-1 cells, which were frozen at passage 12 in 2016. I got the cells back to culture in RPMI + 10% FCS + 1% HEPES + 1% PenStrep. The cells seemed to recover...

19 June 2019 7,802 6 View

How to keep reference in predictor variable and outcome variable in multiple logistic regression analysis by using EPI INFO 7 Software?

HI, I am analyzing data related to a project entitled "Assessment of stigma, fear and discrimination among healthcare workers towards PLWHIV". We are planning to associate socio-demographic...

21 April 2019 1,615 2 View

Can we leverage Artificial Intelligence & Image Processing for Online Inspection of Packed Cases..?

TITLE: Leveraging Artificial Intelligence & Image Processing for Online Inspection of Packed Cases 1.BACKGROUND: Leaves of a crop are threshed and are packed into 200 Kg cases. 10% of these...

06 February 2019 8,322 2 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

No title

Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...

08 February 2021 7,285 1 View

How define one baker unit(BU) is the amount of hydrogen peroxide catalase ?

07 February 2021 2,006 1 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View

What are some common oxidants used to oxidize iron sulfur clusters in proteins for techniques like EPR?

I have used potassium ferricyanide (which is likely the most common) extensively in the past, but I am curious to know if there are other oxidants that work well. I need to generate the oxidized...

29 January 2021 9,803 4 View

Papain Enzyme Assay...?

Hi all, I would like to know how can I estimate the activity of the enzyme, papain, which is used in reactions as crude latex extract of papaya. Please share some procedures if anybody having...

26 January 2021 569 4 View

Where can I find plants pyr-GC-MS data?

25 January 2021 5,403 2 View

Recombinant restriction enzyme: how strong should the corresponding methyltransferase expression level be?

Hello. We are planning expression of a restriction enzyme in E. coli, and wondering how much of the corresponding methyltransferase is needed to be expressed in order to protect the host genome...

23 January 2021 2,096 4 View