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Questions related from Narayana Murthy Kada
I was trying to sequence 16S rRNA gene of a bacteria. The gene is being amplified but unable to sequence it. Sequencing was attempted for 3 times but failed. What might be the reason?
28 May 2016 1,447 8 View
I have cultured a strain of marine bacteria in Nutrient Broth with 2% NaCl with pH adjusted between 2.0-10.0 in individual tubes to check the tolerance of bacteria towards various pH. After 48 hrs...
09 May 2016 1,034 15 View
Please find the attachment of the Blast results of a sequence. I am unable to interpret the best identity among the results as the results give same Query %, E value and % identity with different...
19 November 2014 686 4 View
Can anyone send me a reference on how to extract compounds from bacterial agar plate, as the particular bacteria shows the phenomenon only on agar plate.
11 May 2014 8,030 7 View
I had ordered primers for a PCR. Actual (correct) FW- CCTCTCTCGAACTCCGGAG RW- GTTGTGGAATTACTCGGAGGAA Ordered (incorrect) with a nucleotide 'A' missing at last of reverse primer FW-...
04 May 2014 4,442 61 View
I would like to know if gene coding of a particular enzyme is same for all bacteria, that is, if the sequence of the gene of an enzyme is the same? For example, I would like to screen the...
03 October 2013 4,682 7 View
Is it possible to detect bacterial enzymes by culture based methods? Can anyone share protocols for culture based methods to detect bacterial enzymes?
21 December 2012 4,095 34 View
I am isolating vibrios from a marine source, a few of my strains are not able to grow again during subculture. Can anyone suggest methods to revive them?
25 July 2012 8,153 8 View