Is there some specific drug to induce injury only in muscle fibers without affecting peripheral nerves?

I would like to induce injury in skeletal muscle fibers to study muscle regeneration but I would like to preserve nerve terminals intact. Could it be possible? Is there some specific drug? Thank...

30 October 2020 9,077 4 View

How do I extract data Hazard Ratio and CI from Kaplan-Meier curves?

I am conducting a meta-analysis of Hazard Ratios (HR), however, most of the studies presented only Kaplan Meier curves without the HR and Confidence Intervals (CI). I am extracting data manually...

23 June 2020 6,584 1 View

How can I solubilize HgNO3 in water?

I need to solubilize HgNO3 in water at different concentrations, but I have notice an orange precipitate formation when I dissolved the salt in water. Probably it is mercury oxide. How can I...

28 March 2019 1,277 3 View

Procedure for thawing cryopreserved hpc sample for flow cytometry viability via 7-aad?

Currently our lab performs post thaw viability via trypan blue. We would like to begin using flow cytometry 7-aad for our post thaw viability. Our attempts so far at thawing our samples have led...

22 September 2018 5,396 3 View

Analysing questionnaire with multiple constructs in SPSS

27 May 2017 9,677 5 View

How to account for phylogenetics in variables (phenols, nitrogen, leaf toughness etc) for australian plant families? PGLS? where do you find the data?

I have a data set of chemical and physical traits (and herbivory data) from trees and vines at one of my study sites. Unfortunately, there was no overlap in the families trees vs vines came from...

26 February 2017 3,263 3 View

It is possible to detect (by WB) a membrane receptor, mainly in the cytosolic fraction after a differential centrifugation?

07 December 2015 5,342 3 View

Aside from looking to prototypicality studies for category lists, are there more sophisticated and open-source databases available (i.e. animals)?

I am looking to generate culturally unbiased and medium frequency words to be used in a memory task.

23 March 2015 4,056 3 View

Has anyone detected BDNF in tissue homogenates by ELISA?

08 January 2015 3,081 7 View

What is the effect of Arginine on siRNA binding?

23 July 2014 363 0 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View