I see a clump on my Inserts(Transwell assay), what is it?

Hello, I am working on a Transwell assay, and I have been trying to optimize the assay for a long time, currently, I am working on hCMEC/D3, seeded at 2x10^4 and left for 5 days (the experiment...

11 December 2019 9,074 3 View

Is it possible to analyze colistin sulfate in a pharmaceutical drug by HPLC with UV detection?

Colistin has weak chromopheres, therefore it is usually analyzed by microbilolgy methods and not by HPLC. is it possible to analyze it by HPLC with PDA detector.

31 December 2017 717 0 View

Why PDA detectors can not withstand colored mobile phases in HPLC analysis?

In an HPLC method of analysis, Copper (II) sulfate is used in the mobile phase which makes the mobile phase blue. However this affects adversely the PDA detector where its intensity is...

08 September 2017 315 2 View

Do TLC methods for impurities in pharmaceutical analysis have sufficient accuracy, precision?

In some monographs of pharmacopoeias, TLC method is used for analysis/quantification of impurities. Does this method have adequate accuracy, precision, LOD, LOQ for such trace analysis of...

06 July 2017 2,326 0 View

Is there a difference in the retention time of chlorhexidine acetate, and chlorhexidine hydrochloride in HPLC analysis?

In a method of analysis of chlorhexidine hydrochloride by HPLC, chlorhexidine acetate is used in system suitability. should be there a difference in retention time between chlorhexidine...

03 April 2017 1,441 5 View

How to separate and analyze simultaneously valproic acid and sodium valproate by HPLC?

Valproic acid and sodium valproate when dissolved in the mobile phase and injected into HPLC, they coelute and can not be separated as they become identical in solution. Is there a method for...

02 March 2017 3,739 5 View

What is the maximum concentration of sodium-1decanesulfonate ion pairing regent in reversed phase mobile phase?

02 March 2017 3,341 3 View

What is the function of tetrabutylammonium hydroxide in the separation of isomers of an analyte?

02 March 2017 4,101 5 View

What is the cause of bubbles formed in the mobile phase of trifluroacetic acid solution and acetonitrile mixture mobile phase in HPLC?

01 February 2017 800 9 View

How to get high resolution in cation exchange Liquid chromatography of two basic analytes?

23 January 2017 2,493 5 View

How the wettability of rock sample can be changed by ageing in pure hydrocarbon oils only?

I want to do wettability analysis of hydrocarbon oil (octane)/rock/surfactant solution system. but to do the analysis first I have to make rock surface oil-wet. Since hydrocarbon oil does not have...

27 February 2021 5,383 3 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View

How can Gamma Ray API units be converted to CPS (counts per second)?

Oil and gas companies run gamma ray tool and take readings in API units while Gamma ray is also noted in cps, so how these units can be converted into each other?

24 February 2021 7,545 2 View

How to remove compound ( Europium complex ) from paraffin oil?

How can I remove my compound from paraffin oil? Mistakenly my compound fell in an oil. Compound = Europium complex

22 February 2021 5,667 2 View

When I mix oil and NaOH solution, Why solid layer in the surface is formed?

During my trial for lubricant oil re-refinery process, I use NaOH as coagulant agent. After overnight of gravity settling, a solid layer in the surface was form. what product is that? since the...

22 February 2021 2,116 4 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View

The type of filter paper used to filter an epoxidized oil (the mixture is an epoxidized oil with magnesium sulfate) ?

I have a mixture of epoxidized oil and magnesium sulfate wich type of filtre paper should i use

21 February 2021 3,944 3 View

What reaction will occur when adding hexamine into ammonium nitrate in an acidic medium?

Hello, I am investigating properties of water in oil emulsion explosives. And adding hexamine (hexamethylenetetramine) into one of the phases that are used to make the emulsion should increase the...

21 February 2021 8,680 1 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View