Is there any protocol for myelin coating?

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Rajan Tiwari

https://www.researchgate.net/post/Which_is_the_best_method_for_myelin_staining

Article A simple protocol for paraffin‐embedded myelin sheath staini...

Chapter Staining Methods for Normal and Regenerative Myelin in the N...

https://www.omicsonline.org/isolation-purification-and-verification-of-peripheral-nerve-myelin-derived-from-bovine-cauda-equina-2155-9562.S7-002.pdf

http://histologia.ugr.es/descargas/CTM-T01-2014.pdf

Article A New Method for Intense Staining of Myelin

Jill Miotke

Dear Pranay,

That method is the one that was also used by Marie Filbin's lab to examine myelin inhibition of CNS axonal regeneration, so you can look at some of that lab's papers for further details. For instance, this one is available in open access:

Article Prior Exposure to Neurotrophins Blocks Inhibition of Axonal ...

Or, their previous publication on inhibition:

Article Myelin-Associated Glycoprotein in Myelin and Expressed by Sc...

One recent paper that is readily available is from the Anderson lab at UC Irvine, and has some additional details about processing the myelin:

https://www.ncbi.nlm.nih.gov/pubmed/25762679

Good Luck!

Jill

Pranay Mandal

Thanks a lot Jill Miotke