I have cloned YeGFP in a mammalian expression vector, but I don't see any GFP expressionin HEK cells. Is YeGFP is supposed to fluoresce in mammalian cells?
How do you transfect you HEK cells with the vector?
If I get a piece of DNA, how can I tell if the DNA is from Mitochondria of Bacteria?
08 September 2019 6,986 6 View
I used laminin at a concentration of 10ug/ml for growing mice cortical neurons. Can I store it in -20 and re-use it?
09 October 2018 9,712 1 View
I am extracting myelin fro rat brain following Norton and Poduslo, 1973 method, but I don't know how to use or process the myelin after that. Also, I couldn't find any protocol for plating myelin....
10 November 2017 855 3 View
What concentration of Laminin is ideal for neuronal culture? And should I dissolve it in water or PBS?
08 September 2016 7,550 11 View
I am doing electropration in my neurons with some construct that has been tagged with GFP. Now I want to stain the neurons for Tau protein in red. I am getting very very faint or no staining at...
04 May 2016 7,243 11 View
Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...
03 March 2021 5,999 3 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !
02 March 2021 5,785 3 View
I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...
02 March 2021 9,945 3 View
im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...
02 March 2021 2,246 2 View
I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
01 March 2021 9,310 4 View
Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?
01 March 2021 9,447 3 View
Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...
01 March 2021 1,843 2 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...
01 March 2021 6,142 3 View