I have cloned YeGFP in a mammalian expression vector, but I don't see any GFP expressionin HEK cells. Is YeGFP is supposed to fluoresce in mammalian cells?
How do you transfect you HEK cells with the vector?
If I get a piece of DNA, how can I tell if the DNA is from Mitochondria of Bacteria?
08 September 2019 7,195 6 View
I used laminin at a concentration of 10ug/ml for growing mice cortical neurons. Can I store it in -20 and re-use it?
09 October 2018 9,840 1 View
I am extracting myelin fro rat brain following Norton and Poduslo, 1973 method, but I don't know how to use or process the myelin after that. Also, I couldn't find any protocol for plating myelin....
10 November 2017 976 3 View
What concentration of Laminin is ideal for neuronal culture? And should I dissolve it in water or PBS?
08 September 2016 7,705 11 View
I am doing electropration in my neurons with some construct that has been tagged with GFP. Now I want to stain the neurons for Tau protein in red. I am getting very very faint or no staining at...
04 May 2016 7,364 11 View
I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.
09 August 2024 9,894 2 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...
05 August 2024 3,290 1 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,974 3 View