As the standard, residual DNA in bone allograft should not exceed 40ng DNA/g of dry ECM, So in quallity control part,the residual DNA have to be tested.
I want to know what are the common test in this field?
Hi Although the ladder is sharp but my pcr bands are too fuzzy and like smears. I try to set up one of my genes but it still stands for 3 bands, unfortunately. Tm is 65 but there are still...
17 June 2024 7,412 1 View
Hi, I have done mtt test and realized that my reagent ic50 is 50nM. Do I have to do ally cellular assay by treating with this concentration? Do we do the mtt test for this purpose? Of course I...
10 May 2024 1,314 2 View
Actually as I searched about, there are two major method in determining hydrogen peroxide concentration, one is titration with sodium thiosulfate and other is titration with permangenate, I wanted...
29 April 2024 3,565 4 View
I am a graduate student writing to humbly request your assistance. I am currently working on my thesis which focuses on medical image reconstruction techniques. For my research, I need real...
27 September 2023 1,794 3 View
I used this protocol but I did not answer 1) add 250 µl plasma to 750 µl Triazole 2) 5 min incubation 3) add 150 µl chloroform, mix 12 sec, 3 min incubation 4) Centrifuge at 10,000g for 10 min at...
11 August 2023 4,191 0 View
I have set up a PCR using primers that amplify only the exons of my gene of interest. The template I am using is cDNA (mouse samples). I set up this PCR a year ago with an annealing temperature of...
30 July 2023 256 15 View
in order to quantify the concentration of Glycerol monostearate in aqueous solution by UV spectrophotometer, what is the maximum lambda of Glycerol monostearate?
13 May 2023 4,307 3 View
I work on Platelet and need to some information for culturing. How can activate platelet I mean which activator and how much time? as well as how can be stop this process without platelet...
08 May 2023 2,897 0 View
Hello, I'm currently working on a project in lesion detection and I'm looking for image reconstruction codes for algorithms related to this field.(ASIR. 3DRP. FORE FBP. FORE OSEM. IMR or SAFIRE) I...
19 April 2023 2,712 2 View
19 April 2023 9,065 0 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
In order to show people the beauty of control and enhance enthusiasm for learning control theories, are there any good simple systems or platforms to recommend?
05 August 2024 10,034 1 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
I am currently researching the impact of environmental toxins on children's health and would greatly appreciate insights from experts in the field. If you are an expert or researcher working on...
02 August 2024 4,474 2 View
I have seen plenty of existing works on applied Reinforcement Learning (RL) policies for optimized scheduling in IoT networks including Q-learning, DQNs, and the newer ones including PPO for...
01 August 2024 8,754 2 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
What can you look for to know that the circuit model being used is overfitting the data. How do you know when overfitting is occurring?
31 July 2024 7,425 0 View
Hi researchers! I'm working on soil texture analysis, and the end result for sand is doubtful because there is black sediment appearing after drying, as shown in the figure. Is it considered sand?...
30 July 2024 557 2 View
I am doing research on wear mechanism of the additive manufactured metal parts under dry condition. Please share the reference to start the analytical modelling of the wear prediction.
29 July 2024 6,115 0 View
Why microbes are used in environmental engineering and role of microbes in reducing environmental pollution and improving environmental quality?
25 July 2024 5,192 5 View