In my experience, Hp could grow well in the blood agar in microaerophilic condition for 3-5 days. You can then scrape some of the bacteria to 5% FBS in brucella broth and to adjust OD550nm to 0.2. Then add 1ml Hp in the 100ml 5%FBS brucella broth with shake 200rpm for 24-48hrs.

Since Hp could induce gastritis, peptic ulcer and gastric cancer, it usually better cultured in the Microbiology Department, not in the common lab.

Good luck!

Also do not forget to add selective antibiotics in the medium...say Columbia Blood Agar if you are trying to isolate Hp from gastric biopsies..

You can culture on a common lab using a bunsen burner or a biological safety camera. H. pylori gets easily contaminated so preferably use disposable material.

H. pylori is a fastidious microorganism and requires complex growth media. Often these media are supplemented with blood or serum. These supplements may act as additional sources of nutrients and possibly also protect against the toxic effects of long-chain fatty acids. The latter function may also be performed by more defined medium supplements, such as ᵝ-cyclodextrins or IsoVitaleX, or by using activated charcoal. Commonly used solid media for routine isolation and culture of H. pylori consist of Columbia or brucella agar supplemented with either (lysed) horse or sheep blood or, alternatively, newborn or fetal calf serum. For primary isolation but also routine culture, selective antibiotic mixtures are available, although these are not required per se. The often used Dent supplement consists of vancomycin, trimethoprim, cefsoludin, and amphotericin B, whereas the alternatively used Skirrow supplement consists of vancomycin, trimethoprim, polymyxin B, and amphotericin B. Both selective supplements are commercially available. Liquid media usually consist of either brucella, Mueller-Hinton, or brain heart infusion broth supplemented with 2 to 10% calf serum or 0.2 to 1.0% ᵝ-cyclodextrins, often together with either Dent or Skirrow supplement. Growth of H. pylori in chemically defined media has been reported, but these are not suitable for routine growth and isolation of H. pylori. Most of the commercially available synthetic media, such as tissue culture media, do not support the growth of H. pylori without the addition of serum, perhaps with the exception of Ham’s F-12 nutrient mixture. Isolation of H. pylori from gastric biopsy samples is difficult and not always successful. Cultures should be inspected from day 3 to day 14. H. pylori forms small (1-mm), translucent smooth colonies. Upon successful subculturing, H. pylori isolates tend to adapt to the growth conditions used in the laboratory. Subsequently, good growth can generally be achieved following 1 to 3 days of incubation when reference strains and laboratory-adapted isolates of H. pylori are used. It should be noted that once a culture reaches the stationary phase, the growth rate rapidly declines, accompanied by the morphological change to a coccoid form. Prolonged culture does not lead to any significant increase in colony size but rather leads to a transition to the unculturable coccoid state.

A key feature of H. pylori is its microaerophilicity, with optimal growth at O2 levels of 2 to 5% and the additional need of 5 to 10% CO2 and high humidity. There is no need for H2, although it is not detrimental to growth. Many laboratories utilize standard microaerobic conditions of 85% N2, 10% CO2, and 5% O2 for H. pylori culture. Growth occurs at 34 to 40°C, with an optimum of 37°C.

it can be grown in BHI supplemented with horse blood and yeast extract, the growth conditions are usually 5%o2 and 10% co2 in 37 C. but usually bacteria needs 48 hours to 5 days to grow in the mentioned medium.

Had a question ? Can egg yolk emulsion replace blood ?

Read about this Microcapillary method to grow Helicobacter pylori I think it  is easy, search on google for more details

I need isolates of Helicobacter pylori for plant extract test of susceptibility. Can you assist me by sending them. Please, how do I maintain the isolates in my laboratory. I work in Medical microbiology laboratory of a Teaching hospital.

I found this reference most comprehensive.

Blanchard, T. G., & Nedrud, J. G. (2012). Laboratory Maintenance of Helicobacter Species. Current Protocols in Microbiology, CHAPTER,  Unit 8B.1. http://doi.org/10.1002/9780471729259.mc08b01s24

Working with H. pylori does not require a special laboratory, but it is necessary to pay attention to the sterile conditions.

Moreover; Defined media are not available for the culture of H. pylori; the organism requires complex basal medium like Brucella Agar or Columbia agar (either solid or liquid) with some form of supplementation such as whole blood, heme, serum, charcoal, cornstarch, or egg yolk emulsion. While some of these supplements may serve as nutritional substrates, a key function may be detoxification of the medium and protection of the organisms.

In this regard supplementation of media with cyclodextrins supports excellent growth of H. pylori. Fresh isolates of H. pylori grow best under microaerobic conditions. However, after laboratory passage, some strains become sufficiently aerotolerant that they can grow in 10% CO2. H. pylori grow poorly, if at all, under anaerobic conditions,