What is the composition of NDPKinase buffer plus luciferase assay?

Oleg Suslov @Oleg_Suslov

01 January 2018 1 8K Report

Can you provide the buffer composition for the following reaction:

ADP + dNTP with nucleoside diphosphate kinase?

The outcome of the reaction (ATP) will be detected with the luciferase assay, for example ATPlite.

Thank you.

Oleg

Oleg Suslov

It is really strange answering my own question, but here it is the kinase reaction buffer composition:

40mM Tris (pH 7.5), 20mM MgCl2, and 0.1mg/ml BSA.

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