I plan using HisPur Cobalt resin for the protein purification. What approach, Cobalt Spin column or gravity flow column, gives the better protein purity?
Thank you
Hi there,
If you follow exactly the same protocol in terms of buffer composition then results should be strictly identical.
I don't think there will be a difference except for the time taken.
I do not think that there is a superior one. At the end what matters are your conditions, the selected metallic ion, and your protein.
Thank you guys. I really appreciate your help.
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