Oleg Suslov

4 Questions 18 Answers 0 Followers

Questions related from Oleg Suslov

Cobalt Spin column vs. gravity flow, what approach is superior?

I plan using HisPur Cobalt resin for the protein purification. What approach, Cobalt Spin column or gravity flow column, gives the better protein purity? Thank you

03 March 2018 2,716 4 View

What is the composition of NDPKinase buffer plus luciferase assay?

Can you provide the buffer composition for the following reaction: ADP + dNTP with nucleoside diphosphate kinase? The outcome of the reaction (ATP) will be detected with the luciferase assay, for...

01 January 2018 8,022 1 View

LFIA question. Correct load technique for test and control lines.

How to load manually streptavidin-biotin-aptamer solution on the test and control lines in order to achieve the thin line without damaging the filter? I am using Prima 40; 2 microL per line. When...

01 January 2017 5,666 0 View

is there a method for aptamer-gold conjugation?

What is more efficient method of conjugation of an aptamer to 40nm gold nanoparticles: using the thiolated aptamer or polyA one? Thank you.

11 November 2015 6,353 8 View