There are some suppliers (Hi media etc..) are manufacturing plasmid isolation kit for endotoxin removal, so use those kits.

all the best

I'd recommend to use Invitrogen PureLink endofree kits - up to Giga scale:

https://www.thermofisher.com/order/catalog/product/A31232

depends on the column resin. Polymyxin B and Tritons (eg X100) are most popular

Hello,

Endotoxin can be removed with three possible mechanisms:

1. Affinity based : Affi-Prep Polymyxin resin from Bio-rad

http://www.bio-rad.com/en-in/product/affi-prep-polymyxin-resin?ID=5c53a1f3-b0da-4799-8a54-7e94f090e0f9

2. Charge based : MacroPrep High Q ( When your Protein have basic pI and operating at neutral pH) , You can try Ceramic Hydroxy Apatite also.

Selecting right resin for endotoxin removal :

http://www.bio-rad.com/webroot/web/pdf/lsr/literature/Bulletin_6813.pdf

3. Size based : If your desired protein size less then 5kda then you can try TFF (10kda) in flowthrough (permeate mode), Endotoxin have higher size so endotoxin will retain in retenate and your product will come in flow-through.

Hope this is useful.

Please contact me for any more info or support.

Hello, Endotoxin Removal solution from sigma can be used for endotoxin removal.

See SDS here: https://www.nwmissouri.edu/naturalsciences/sds/e/Endotoxin%20removal%20wash.pdf

Hi Jenifer,

The web link in your lasted answer doesn't work now. Do you have the SDS pdf file and if yes, could you share the file? Thanks in advance. https://www.nwmissouri.edu/naturalsciences/sds/e/Endotoxin%20removal%20wash.pdf