Nageswar Reddy Manchala

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Questions related from Nageswar Reddy Manchala

Can we use quantitative gene expression levels without reference dye in qPCR MASTER MIX?

Hello I had a small question regarding the reference dye that incorporated in qPCR master mix of 2x Syber green or Taq Man mix. Generally these reference dyes such as ROX is incorporated for the...

31 August 2018 9,411 0 View

Did any one used autophagy dye for meausring autophagy induction?

I tried to see the autophagy formation in the Rapamycin treated cells by using autophagy dye.Did any one tried by measuring fluorescence of autophagy dye added to the cells after induction?

30 November 2014 2,829 8 View