Dear Mohamed,

it will depend on several factors, but in long term choose the instrument you have available in your lab (that way in case you need to repeat your analysis it'll be quicker to book and run your sample or in case you have questions you can always ask the technician for guidance); choose the method that requires less sample preparation (that way less chance of introducing contaminants); and choose the method that gives you the best results for the shortest analysis time (that way more samples can be processed giving validity to your conclusions).

Nowadays, LC-MS/MS has become a popular technique that allows you to decipher the molecular details, but unless you're familiar with the technique the GC-MS/MS might be the best choice as you'll have access to a very complete database for compound confirmation. Hope this helps, Ana 

here some that I found off the internet

LC-MS/MS have several advantages over GC-MS/MS and specially in case of hormones the former is always preferred, however agree to Ana that choice of selection also depends on availability of instruments in your lab.  

My choice is LC/MS/MS because most GC methods require derivitization first. GC methods are also generally longer.  One down fall of LC/MS/MS is proper ionization in the source can be problemattic depending upon your inlet source.  Photoionization sources work well for steroids but is a rare inlet source.

Thank you all for your help

I would like to find a method for analysis of 17 beta estradiol by GC-MS/MS which i have experience in using it.

derivatization is not a big problem for me because i am using internal stander.

the method must be applicable in meat liver kidney and fat matrix.

Analyst. 2000 Apr;125(4):711-4.

Determination of estrone and 17 beta-estradiol in human hair by gas chromatography-mass spectrometry.

Choi MH1, Kim KR, Chung BC.

Author information

Abstract

An efficient procedure is described for the determination of estrone and 17 beta-estradiol in hair by gas chromatography-mass spectrometry (GC-MS). The method involves alkyloxycarbonylation with isobutyl chloroformate (isoBCF) of phenolic hydroxy groups after alkaline digestion of hair samples. The resulting isobutyloxycarbonyl derivatives of estrone and 17 beta-estradiol are extracted with hexane and subjected to chlorodifluoroacetyl derivatization in order to protect the remaining alcoholic hydroxy groups. When GC-MS with selected ion monitoring (SIM) was used, the quantitative ions were at m/z 270 and 384 in the electron ionization mass spectra for estrone and 17 beta-estradiol, respectively. The detection limits for SIM of the steroids were 1 and 2 pg, respectively, and the SIM responses were linear with correlation coefficients varying from 0.991 to 0.994 in the concentration range 0.2-4.0 ng g-1 for the estrogens studied. The detection of estrone and 17 beta-estradiol in hair samples was possible in the concentration range of 0.24-1.30 ng g-1. The concentrations of the two estrogens detected were different in male and female hair samples.