You may use Actinomyces isolation agar.
http://himedialabs.com/TD/M490.pdf
Good luck
Best Regards
We are routinely using Ken Night agar media for isolation of actinomycetes and getting good results.
A number of culture media are there for isolation of Actinomyces.
1. Glucose asparagine agar medium (Krinsky, 1941)
2. Arginine- Glycerol medium (El-Nakeeh and Lechevailer, 1963)
3. Tyrosine agar (Shinobu, 1958)
etc. You may try.
Actinomyces may be cultured on Blood agar (BA) with sheep blood. Of course these microbes are anaerobic (A. israeli, ), so keep that in mind. Under the first link you'll find an article how it may look on BA.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2446836/
Furthermore, check these two links I append below:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2950629/
http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.319.3529&rep=rep1&type=pdf
These papers are on media to culture Streptomyces species, although they may be useful also for Actinomyces. I've read somewhere that for Actinomyces you may use YEME agar without glucose. Concluding from their habitats (e.g., mouth) rich media should do.
Good luck with your experiments!
thioglycollate broth as enrichment medium
blood agar can be used. Anaerobic culture is preferred as this is microaerophilic organism
in Thioglycolate broth cottony wool like growth after 48 hours incubation at 37 0c
On blood agar-early growth will be like spider web after 48 hours , molar tooth appearance is seen
You may use 5% sheep blood and chocolate agars, CNA, and HBT incubated at 35° to 37°C in 5% to 10% carbon dioxide (CO2) within 48 hours of inoculation. Keep in mind that although most Actinomyces spp. are facultative anaerobes, some relevant species are strictly anaerobic, so cultures must be incubated in an anaerobic atmosphere.
You can also use semi-selective media such as mupirocin-metronidazole blood agar to increase isolation rates. It can inhibit overgrowth of concomitant organisms. Other enriched media that can be used for Actinomyces isolation also includes Brucella Blood Agar with hemin and vitamin K1 and brain heart infusion broth.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4094581/
The article below may also help you acquire and isolate Actinomyces from your mouth. The researchers isolated Actinomyces in human saliva.
https://aem.asm.org/content/aem/47/5/901.full.pdf
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