Pooja Khurana

13 Questions 24 Answers 0 Followers

Questions related from Pooja Khurana

Do mouse embryonic fibroblasts express pluripotency markers?

Hello, I am doing flow cytometry to evaluate the pluripotency of mouse embryonic stem cells (mESCs) which have been derived and cultured on mouse embryonic fibroblasts (MEFs) Unfortunately, I am...

01 June 2017 2,461 4 View

What is the best way to induce neural differentiation of mouse embryonic stem cells?

Hi, I have been trying to induce neural induction in my mESC lines by using -DMEM-F12 + N2+B27+NEAA+ bFGF. Although I started getting rosette formation from day5/6 onward, and mature neural marker...

01 December 2016 6,051 3 View

How to do Karyotyping of mouse embryos?

Hello, I am interested in Karyotyping (just chromosome number) of mouse embryos-blastocysts. I think it wont be too different from human embryos Karyotyping. Could anyone please share their...

06 July 2016 2,849 4 View

What are the earliest differentiation markers for mouse embryonic stem cells?

Hi, I am interested in doing pluripotency analysis of my mESCs, and want to include the earliest markers for differentiation. I do observe some form of differentiation in my colonies, but not sure...

05 July 2016 1,946 5 View

What are the best assays to assess PFK activity in mouse embryonic stem cells?

I am trying to assess the activity of PFK enzyme in mESC lines. Recently in the metabolomics analysis, I discovered that although the cells show an increased uptake of glucose 6-P and fructose...

15 February 2016 1,981 0 View

Any advice for tissue culture contaminant detection kit?

Hi, I am looking to buy a kit for earliest detection of bacteria and fungus in cell culture (mESCs/ MEFs). I know these contaminants are easily detected by eye/ under the microscope. But I've been...

17 December 2015 3,571 7 View

What are the earliest signs of fungal infection/ tests one could perform to prevent the spread of infection in cell culture?

We have been facing recurring fungal infections in our mouse fibroblast cultures and hence in stem cells that we thaw onto the MEF cells. I have tried decontaminating the incubator, clean the...

05 August 2015 4,642 7 View

Is there any relation between Claudin 6 and the development of metabolic diseases?

Hi, I was just wondering how tight junctions contribute to the development of diseases and if there is any known connection between Claudin 6 expression in developmental programming and/or the...

04 June 2015 8,804 2 View

How do I differentiate between MEFs and mESCs?

Hi, which antibody/marker acts best to distinguish between MEFs and mESCs in flow cytometry when using Annexin V-PI for Apoptotic and also for PI only cell cycle analysis? Considering that Annexin...

03 June 2015 6,117 4 View

Has anyone worked with the R&D Systems Mycoprobe Kit for Mycoplasma detection?

Hi has anyone had any issues using this kit? My negative control of the kit gives a higher value than the values suggested for positive control. Both controls from the kit itself. Tested for the...

13 November 2014 1,047 1 View

C-band staining protocol for karyotyping of mESCs?

Hi, I want to karyotype my mESC lines for characterization. I used Giemsa staining earlier, without arresting my cells in metaphase, therefore now finding it quite hard to find countable...

15 July 2014 1,059 10 View

How to culture mouse Blastocysts after they hatch and attach on mitomycin treated MEFs?

When I culture my mouse embryos at E 3.5, I let them stay on MEF coated dishes for 2-3 days, till they hatch and attach. I then trypsinize them and dissociate, later transfer to 96-well plates to...

06 March 2014 7,041 6 View

Can anyone please suggest protocols to culture mESCs on MEFs?

Please suggest protocols to culture mESCs on MEFs directly after flushing embryos at 3.5dpc (early blastocyst stage). Thanks!

24 January 2014 7,491 1 View