14 Questions 26 Answers 0 Followers
Questions related from Pooja Khurana
Hello, I am doing flow cytometry to evaluate the pluripotency of mouse embryonic stem cells (mESCs) which have been derived and cultured on mouse embryonic fibroblasts (MEFs) Unfortunately, I am...
01 June 2017 2,469 4 View
Dear all, while SB431542 is established to block TGFbeta and LDN193189 to inhibit the BMP pathway, I was wondering if anyone has seen the combined effect of the two in neural differentiation of...
15 February 2017 8,510 1 View
Hi, I have been trying to induce neural induction in my mESC lines by using -DMEM-F12 + N2+B27+NEAA+ bFGF. Although I started getting rosette formation from day5/6 onward, and mature neural marker...
01 December 2016 6,060 3 View
Hello, I am interested in Karyotyping (just chromosome number) of mouse embryos-blastocysts. I think it wont be too different from human embryos Karyotyping. Could anyone please share their...
06 July 2016 2,861 4 View
Hi, I am interested in doing pluripotency analysis of my mESCs, and want to include the earliest markers for differentiation. I do observe some form of differentiation in my colonies, but not sure...
05 July 2016 1,954 5 View
I am trying to assess the activity of PFK enzyme in mESC lines. Recently in the metabolomics analysis, I discovered that although the cells show an increased uptake of glucose 6-P and fructose...
15 February 2016 1,989 0 View
Hi, I am looking to buy a kit for earliest detection of bacteria and fungus in cell culture (mESCs/ MEFs). I know these contaminants are easily detected by eye/ under the microscope. But I've been...
17 December 2015 3,588 7 View
We have been facing recurring fungal infections in our mouse fibroblast cultures and hence in stem cells that we thaw onto the MEF cells. I have tried decontaminating the incubator, clean the...
05 August 2015 4,650 7 View
Hi, I was just wondering how tight junctions contribute to the development of diseases and if there is any known connection between Claudin 6 expression in developmental programming and/or the...
04 June 2015 8,831 2 View
Hi, which antibody/marker acts best to distinguish between MEFs and mESCs in flow cytometry when using Annexin V-PI for Apoptotic and also for PI only cell cycle analysis? Considering that Annexin...
03 June 2015 6,123 4 View
Hi has anyone had any issues using this kit? My negative control of the kit gives a higher value than the values suggested for positive control. Both controls from the kit itself. Tested for the...
13 November 2014 1,054 1 View
Hi, I want to karyotype my mESC lines for characterization. I used Giemsa staining earlier, without arresting my cells in metaphase, therefore now finding it quite hard to find countable...
15 July 2014 1,066 10 View
When I culture my mouse embryos at E 3.5, I let them stay on MEF coated dishes for 2-3 days, till they hatch and attach. I then trypsinize them and dissociate, later transfer to 96-well plates to...
06 March 2014 7,049 6 View
Please suggest protocols to culture mESCs on MEFs directly after flushing embryos at 3.5dpc (early blastocyst stage). Thanks!
24 January 2014 7,500 1 View
