Pooja Khurana

7 Questions 13 Answers 0 Followers

Questions related from Pooja Khurana

What is the best way to induce neural differentiation of mouse embryonic stem cells?

Hi, I have been trying to induce neural induction in my mESC lines by using -DMEM-F12 + N2+B27+NEAA+ bFGF. Although I started getting rosette formation from day5/6 onward, and mature neural marker...

01 December 2016 6,008 3 View

Any advice for tissue culture contaminant detection kit?

Hi, I am looking to buy a kit for earliest detection of bacteria and fungus in cell culture (mESCs/ MEFs). I know these contaminants are easily detected by eye/ under the microscope. But I've been...

17 December 2015 3,508 7 View

What are the earliest signs of fungal infection/ tests one could perform to prevent the spread of infection in cell culture?

We have been facing recurring fungal infections in our mouse fibroblast cultures and hence in stem cells that we thaw onto the MEF cells. I have tried decontaminating the incubator, clean the...

05 August 2015 4,593 7 View

Is there any relation between Claudin 6 and the development of metabolic diseases?

Hi, I was just wondering how tight junctions contribute to the development of diseases and if there is any known connection between Claudin 6 expression in developmental programming and/or the...

04 June 2015 8,703 2 View

Has anyone worked with the R&D Systems Mycoprobe Kit for Mycoplasma detection?

Hi has anyone had any issues using this kit? My negative control of the kit gives a higher value than the values suggested for positive control. Both controls from the kit itself. Tested for the...

13 November 2014 985 1 View

C-band staining protocol for karyotyping of mESCs?

Hi, I want to karyotype my mESC lines for characterization. I used Giemsa staining earlier, without arresting my cells in metaphase, therefore now finding it quite hard to find countable...

15 July 2014 996 10 View

Can anyone please suggest protocols to culture mESCs on MEFs?

Please suggest protocols to culture mESCs on MEFs directly after flushing embryos at 3.5dpc (early blastocyst stage). Thanks!

24 January 2014 7,433 1 View