What is the coating used for HMC3 human microglia cell line? I couldn't find it anywhere?

11 March 2024 1 9K Report

I got the cells from ATCC and I want to plate them and start culturing. So far in anywhere I couldn't find which type of coating needed for the flasks, if ever needed?

Aaron Dhanda

I believe you could use Poly D lysine

Badges
Science topic

More Buse Özbaykent's questions See All

Extracellular vesicle characterisation using immunostaining?

Hello dearest EV people! I want to plate my isolated EVs on coated glass coverslips and image them with confocal microscopy for CDs and other EV markers. I wonder if anyone tried a normal IF...

21 March 2024 3,344 1 View

Is there a scientific explanation ? About cDNA ?

Good work to everyone, cDNA synthesis from RNA with reverse transcriptase reaction is a simple method and then gene expression will be checked with qPCR. After cDNA synthesis, we can store it at...

14 March 2024 7,319 2 View

How long after can I do DNase treatment in by phenol-chloroform extraction method isolated RNAs?

For RNA isolation, I use the phenol-chloroform extraction method. To avoid DNA contamination I've used the DNaseI enzyme. But I'm not sure how long after I can do the DNase treatment. Should I do...

06 January 2024 5,462 3 View

Does anyone know any distribution maps websites/online databases for animal species?

Hello, I'm looking for as many as possible for my bachelor's degree project/thesis. The ones I have found so far are:...

24 January 2023 6,802 1 View

What are these formations in Feline adipose-derived mesenchymal stem cell suspension?

Hi eveyone, This image is feline adipose-derived mesenchymal stem cell suspension. It becomes like this two hours after preparing the product (It is stored at +4 degrees). Viability of stem cells...

14 September 2022 1,792 1 View

How can I fill pdms cracks in microfluidic device inputs?

The punch holes on the microfluidic device are troublesome due to the punch tool, and when we connect the pipes to the device, when we give the flow, cracks occur in the input holes and the device...

24 December 2021 5,010 4 View

How to comment after finding IC50 according to MTT results?

How is the IC50 interpretation made according to the results of the MTT test used to investigate the viability effect of plant extracts on cancer cells?

23 September 2021 7,051 3 View

How to fix free-standing polymer samples on Si-wafer for Atomic Force Microscopy?

Hello, I would like to perform an AFM measurement to my free-standing, polymer-based composites. I have found some special adhesives for this purpose on the internet, however we do not have any...

26 February 2018 7,032 1 View

Adhesion strength of coating?

How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...

10 August 2024 942 3 View

How do I conformally do PR spin coating on trench structure?

I did PR spin coating on trench structure. I used AZ P4620 PR and the thickness or PR is around 11um. The substrate is Si. And my trench structure depth is 141um(negative way). Even though I...

08 August 2024 2,298 0 View

Is changing the medium during the experiment overestimate the results?

I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...

07 August 2024 2,283 2 View

Why do i have a weak plasma and no coating during rf sputtering?

I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.

07 August 2024 4,526 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Can you recommend a 96-well plate for minimal cell adherence to the wells during phagocytosis experiments?

I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...

04 August 2024 5,228 4 View

PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View

Found this object in the cell culture - do you have any suggestions what it might be?

Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...

01 August 2024 2,250 2 View

Publishing a checklist?

Does anybody know of entomological Journals without page charges that would publish an extensive checklist (Hong Kong Aculeates wasps) covering 350 species (of which more than half are new to Hong...

01 August 2024 6,134 2 View

What is the reason I get 400nm nanoparticles using an extruder with 200 nm membrane for cell membrane coating?

I am trying to coat my micelles and PLGA based nanoparticles with cell membrane using an Avanti mini extruder according to literature . I use 200nm membrane but my particles end up to be 400nm And...

30 July 2024 3,294 3 View