Extracellular vesicle characterisation using immunostaining?

22 March 2024 1 3K Report

Hello dearest EV people!

I want to plate my isolated EVs on coated glass coverslips and image them with confocal microscopy for CDs and other EV markers. I wonder if anyone tried a normal IF staining method on isolated EVs? So far I only saw PEG method published for this...

Thank you in advance!

Marius Pollet

Hey Buse, you could also use dPCR to detect CDs and other markers on the EV surface. I guess that is easier compared to staining, and you will not need too much antibody for it.

You can find the application note for it here: https://www.actome.de/downloads/Actome_EV_Quantification_App_Note.pdf

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