How is the IC50 interpretation made according to the results of the MTT test used to investigate the viability effect of plant extracts on cancer cells?
Hello Buse Tepe
An IC50 measurement tells you about the concentration at which a drug can inhibit a particular biological process by 50%. For example, in this case, IC50 is the dose of the plant extract which inhibits the proliferation of cells by 50%.
IC50 is most widely used and provides information of a drug's efficacy and a measure of potency. The lower the IC50 value the more potent is the plant extract. For a good candidate compound the IC50 value should be less than 10 micromolar.
Best.
Hello ma'am,
As you have already got the absorbance in MTT analysis, calculate the Cytotoxicity by dividing the Average by the Control value.
Otherwise, you can get the Cell Viability = (Control-Average)/Control
And get The % Cell viability as well.
When you get all the percentiles compared with Control values put them in Graphpad prism for getting the graph of % cytotoxicity and % of cell viability.
IC50 is the value where 50% cells die due to the drug application.
Which drug shows IC50 in lowest concentrations, thats called the best and safe drug to use compared to others.
Thanks,
Dear Buse,
If you have already calculated IC50, you may just go on and say that in you manuscript. Something like: "The IC50 value was determined to be...".
However, if you want to explain what an IC50 is, remember that it is a context-dependent term. It simply means the half maximal inhibitory concentration and in you case inhibitory effect is the "inhibition of viability". Since what you measure is not cell death, it does not give you the concentration which kills half of the cells. MTT cannot tell you what happened to the cells, it can only tell you the amount of viable cell at your endpoint. Viability may decrease due to proliferation rate inhibition or due to cell death. Since you cannot know which, I don't recommend you to comment on those. Cytotoxicity, proliferation rate and viability are not the same thing. For more information on this I recommend our paper on this topic: https://www.biorxiv.org/content/10.1101/2021.04.12.439525v1
Accordingly, you may say something like "x extract inhibited viability of y cells by 50 percent at..."
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