Good work to everyone,
cDNA synthesis from RNA with reverse transcriptase reaction is a simple method and then gene expression will be checked with qPCR. After cDNA synthesis, we can store it at +4 degrees for a short time. We keep it at -20 degrees for a long time. My question is; which cDNA gives more optimum results in quantitative PCR? Is it cDNA that is kept at -20 degrees for 1 or 2 days, or is it cDNA that is kept at +4 degrees for 1 or 2 hours ? Is there a scientific explanation for that reaction ?
DNA should be quite stable at 4 ˚C, so I would expect that those samples would be better as freezing and thawing samples will induce some degradation. However, both of these conditions should give results that are virtually identical as they are not being stored for extended periods of time.
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