We culture cells on a microchip with a membrane that has autofluorescence and after few hours the stained cells start fading I am searching for a cell tracker dye with strong intensity to make it easy to track cells in this high noise background.
If the visualization of the nucleus is enough information for you, then you can try Hoescht 33342. It works on living cells and I don't know anything with a brighter signal than Hoescht. As a consequence, you'll also be able to identify the mitotic status of the cells...
http://cshprotocols.cshlp.org/content/2011/1/pdb.prot5557.full
I am doing experiment for Studying cell migration, is this dye could work?
Well then you could consider the center of the nucleus as the center of the cell... But why not using simple phase contrast or DIC ?
http://micro.magnet.fsu.edu/primer/techniques/dic/dicphasecomparison.html
If the answer is "because cells are too crowded", then Hoescht 33342 would be even more reliable than any membrane or cytosolic dye since two nuclei will always be easily resolved. However, for certain cells types (neurons), the center of the cell may not always be the center of the nucleus...
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