I am doing cell migration assay for MD MB231 on a microchip using collagen to coat the surface, cells look healthy and attached to the collagen but they don't show migration after 12 hrs. How could I make them migrate without stimulant?
I suggest you to use fibronectin or vitronectin coated surface, instead of collagen, to study MDA-MB-231 migration.
I want to know which software that could make a scattered graph like that?
07 August 2018 6,754 2 View
We culture cells on a microchip with a membrane that has autofluorescence and after few hours the stained cells start fading I am searching for a cell tracker dye with strong intensity to make it...
09 October 2017 9,174 3 View
08 September 2017 7,643 1 View
I want to make coupling reaction between a compound with carboxylic group and the aminethat have tetrazine. I used HATU and DIPEA for the coupling for overnight. I get three spots may be the...
05 June 2017 9,431 2 View
I want to know if the tetrazine is table or not in acidic or basic medium.
04 May 2017 903 0 View
I will react PEG-NH2 with a small compound that has carboxylic acid group using a coupling agent. But I just wondering How could Purify the product?
03 April 2017 2,811 2 View
I want to make a chain from magnetic microbeads
11 December 2016 805 3 View
Proximity effect definition and example
08 September 2016 3,078 0 View
I want to know the detailed mechanism of cis platin and carboplatin with references please
08 September 2016 1,994 2 View
01 February 2016 6,387 4 View
02 March 2021 3,060 3 View
Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...
02 March 2021 2,616 1 View
Is the electropolymerisation of polyaniline coating over stainless steel (SS) via Cyclic voltammetry technique is really adherant? Else how to improve the adhesion of the coating, whether any...
01 March 2021 8,220 2 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...
01 March 2021 6,999 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View
I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....
28 February 2021 7,967 3 View