I am doing cell migration assay for MD MB231 on a microchip using collagen to coat the surface, cells look healthy and attached to the collagen but they don't show migration after 12 hrs. How could I make them migrate without stimulant?
I suggest you to use fibronectin or vitronectin coated surface, instead of collagen, to study MDA-MB-231 migration.
I want to know which software that could make a scattered graph like that?
07 August 2018 6,813 2 View
We culture cells on a microchip with a membrane that has autofluorescence and after few hours the stained cells start fading I am searching for a cell tracker dye with strong intensity to make it...
09 October 2017 9,236 3 View
I synthesized a membrane using a tri-acrylate and PEG and want to measure the pore size and to if this membrane will be permeable for different molecular weight dyes or not?
08 September 2017 7,722 1 View
I want to make coupling reaction between a compound with carboxylic group and the aminethat have tetrazine. I used HATU and DIPEA for the coupling for overnight. I get three spots may be the...
05 June 2017 9,535 2 View
I want to know if the tetrazine is table or not in acidic or basic medium.
04 May 2017 977 0 View
I will react PEG-NH2 with a small compound that has carboxylic acid group using a coupling agent. But I just wondering How could Purify the product?
03 April 2017 2,884 2 View
I want to make a chain from magnetic microbeads
11 December 2016 875 3 View
I want to know the detailed mechanism of cis platin and carboplatin with references please
08 September 2016 2,098 2 View
Proximity effect definition and example
08 September 2016 3,167 0 View
I need references
01 February 2016 6,463 4 View
How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...
10 August 2024 942 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I did PR spin coating on trench structure. I used AZ P4620 PR and the thickness or PR is around 11um. The substrate is Si. And my trench structure depth is 141um(negative way). Even though I...
08 August 2024 2,298 0 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.
07 August 2024 4,526 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View