How I could make a scattered graph for single cell analysis like this?

I want to know which software that could make a scattered graph like that?

07 August 2018 6,754 2 View

What is the cell tracker dye with the highest fluorescence?

We culture cells on a microchip with a membrane that has autofluorescence and after few hours the stained cells start fading I am searching for a cell tracker dye with strong intensity to make it...

09 October 2017 9,174 3 View

How could I measure the permeability of a membrane or the pore size?

08 September 2017 7,643 1 View

What is the best condition for MD MB231 to migrate?

I am doing cell migration assay for MD MB231 on a microchip using collagen to coat the surface, cells look healthy and attached to the collagen but they don't show migration after 12 hrs. How...

08 September 2017 5,584 1 View

What is the effect of the base on tetrazine?

I want to make coupling reaction between a compound with carboxylic group and the aminethat have tetrazine. I used HATU and DIPEA for the coupling for overnight. I get three spots may be the...

05 June 2017 9,431 2 View

How could I purify PEG conjugated Products?

I will react PEG-NH2 with a small compound that has carboxylic acid group using a coupling agent. But I just wondering How could Purify the product?

03 April 2017 2,811 2 View

How to make a chain of magnetic microbeads?

I want to make a chain from magnetic microbeads

11 December 2016 805 3 View

What is the proximity effct in organic chemistry?

Proximity effect definition and example

08 September 2016 3,078 0 View

What is the mechanism of action of Carboplatin as anticancer?

I want to know the detailed mechanism of cis platin and carboplatin with references please

08 September 2016 1,994 2 View

What is the difference between hydroxamic acid derivatives and carboxylic acid derivatives in metal chelation and chelation with metalloenzymes ?

01 February 2016 6,387 4 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View