How to make a chain of magnetic microbeads?

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How I could make a scattered graph for single cell analysis like this?

I want to know which software that could make a scattered graph like that?

07 August 2018 6,813 2 View

What is the cell tracker dye with the highest fluorescence?

We culture cells on a microchip with a membrane that has autofluorescence and after few hours the stained cells start fading I am searching for a cell tracker dye with strong intensity to make it...

09 October 2017 9,236 3 View

How could I measure the permeability of a membrane or the pore size?

I synthesized a membrane using a tri-acrylate and PEG and want to measure the pore size and to if this membrane will be permeable for different molecular weight dyes or not?

08 September 2017 7,722 1 View

What is the best condition for MD MB231 to migrate?

I am doing cell migration assay for MD MB231 on a microchip using collagen to coat the surface, cells look healthy and attached to the collagen but they don't show migration after 12 hrs. How...

08 September 2017 5,656 1 View

What is the effect of the base on tetrazine?

I want to make coupling reaction between a compound with carboxylic group and the aminethat have tetrazine. I used HATU and DIPEA for the coupling for overnight. I get three spots may be the...

05 June 2017 9,535 2 View

What is the stability of tetrazine in basic medium and acidic medium?

I want to know if the tetrazine is table or not in acidic or basic medium.

04 May 2017 977 0 View

How could I purify PEG conjugated Products?

I will react PEG-NH2 with a small compound that has carboxylic acid group using a coupling agent. But I just wondering How could Purify the product?

03 April 2017 2,884 2 View

What is the mechanism of action of Carboplatin as anticancer?

I want to know the detailed mechanism of cis platin and carboplatin with references please

08 September 2016 2,098 2 View

What is the proximity effct in organic chemistry?

Proximity effect definition and example

08 September 2016 3,167 0 View

What is the difference between hydroxamic acid derivatives and carboxylic acid derivatives in metal chelation and chelation with metalloenzymes ?

I need references

01 February 2016 6,463 4 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View