During direct regeneration of Chickpea, the shoot tips start to get brown just after a few day of sub-culturing to multiple shooting medium or to rooting medium and stop responding. What may be the reason?
What is the solution to this problem?
This "browning" or "blackening" as usually referred is due to the accumulation of phenolic compounds, principally because of the activation of the secondary metabolism due to the stress that in-vitro culture is causing to the explants.
For solving this problem there are many things you can try... I leave a link that may help
That is because of some metabolisms which normally happens with Fabaceae family mainly chickpeas. Sometimes you can have a lemon juice and add some drops to the media that should help.
good luck.
I do not know what medium are you using? The browning/burning of the tip is due to stress. Add a lower concentration of GA3 in your medium and see te result
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