I face contamination problems in cell based studies. I was trying to sterilize by UV for 20 mins.
I work with chitosan. I always sterilize chitosan solution using regular steam sterilizer for 30 minutes at 121 C
Ghulam .... thanks for the info .....
Gama sterilization is mostly used for hydrogel sterilization. you can also try steam sterilization or membrane sterilization. This is also efficient method and also handful method.
Hi, i am conducting a Monte Carlo simulation for my new distribution but the MSE values are increasing while the sample size increase, does any experience this issue before and what should i do...
22 February 2021 3,680 4 View
the samples are not completely soluble - just swelled the solvents that's mean the sample may be partially soluble any suggestion, please
16 February 2021 8,801 3 View
There are a few papers dealing with the effects of climate change on the pollination of European orchids, but I wonder if similar studies have been carried out in the Tropics.
04 February 2021 1,818 4 View
Dear all, I am currently writing my master thesis where I am analysizing my data using path analysis and SEM in R (lavaan package). Now that I like to write up my results, I am struggling with...
04 February 2021 8,993 2 View
Dear colleagues: I have been trying to establish a few HP-TLC methods in the lab. In general, for quantification purposes, I prefer CuSO4+acid charring, to primuline staining. Lately I have been...
20 January 2021 9,019 1 View
I need to simulate a mushroom FSS and I'm struggling with the ports. What's the correct way to define them? Should I construct a proper SMA connectors, or is it enough to put a waveguide ports...
19 January 2021 6,590 4 View
i am working in transfer of images between two computer . I don't know how to make connection between Matlab and visual studio . i used a visual basic for programming my controller. Can anyone...
31 December 2020 6,708 4 View
While doing amplification with Primers ( which already gave good amplification in same genus), it fails many time, eventhough the extraction has enough amont of DNA.
31 December 2020 5,649 4 View
The last decade witnessed a tremendous use of computational power for various applications such as deep learning, MD simulations, and astrophysics. Is quantum computers really going to take the...
31 December 2020 1,396 2 View
sector A sample is 313, and sector B sample is 200. If I take sector B population in the whole country then sample B's comes = 330 Q. Can I take sector A in 5 cities and sector B in the whole...
27 December 2020 6,930 2 View
effect of double bond on the flexibility of polymers
07 April 2021 0 0 View
I am writing to request some information for making polymer color flakes. I would appreciate the help.
17 March 2021 0 0 View
I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...
03 March 2021 7,540 4 View
The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...
03 March 2021 4,107 4 View
I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...
02 March 2021 9,945 3 View
Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?
01 March 2021 8,087 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
Its effect appears in real part of ac conductivity also. Can anyone suggest any reason behind it?
26 February 2021 3,487 3 View
24 February 2021 2,362 5 View