19 September 2019 0 8K Report

To stain TMRM in the whole brain of adult Drosophila, I referenced several methods, ultimately following the method below:

1. Dissect 3 adult brains in cold Drosophila Schneider media (DSM)

2. Incubate in 200nM TMRM with DSM - for 40min at RT

3. Mount samples using 50nM TMRM with DSM for Imaging

However, I could not achieve the staining quality like that of the attached image.

So, I changed the method and tried out various experimental conditions:

I tried TMRM concentration of 400nM and 600nM, TMRM incubation temperature of 4℃, and TMRM incubation time of 15min and 1hour.

But, the results were the same.

How can I solve this problem?

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