Look at this link

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC480427/pdf/jclinpath00070-0009.pdf

And thesearticles

Lendrum A C, Fraser D S, Slidders W and Henderson R. (1962)

Studies on the character and staining of fibrin.

Journal of clinical pathology, v. 15, p. 401.

McManus, J.F.A. and Mowry, R.W., (1960),

Staining methods, histologic and histochemical,

Harper & Row, New York, NY, USA.

Histological demonstration techniques, (1974)

Cook, H C.

Butterworths, London, England

m5migu4zj3pb.cloudfront.net/manuscripts/25000/25074/JCI0625074sd.pdf

This method is taken from above reference and may be helpful

Histochemical stain for fibrin

A modification of Carstairs' method to distinguish platelets and fibrin inhistological sections was used to detect fibrin (orange-red), red blood cells(yellow), and collagen (bright blue). Tissue sections were hydrated, stained in 5%ferric alum for 5 minutes, washed in tap water and stained inMayer'shemotoxylin solution for 5 minutes. After washing intap water, the sections werestained in picric acid-orange G solution for 40 minutes and rinsed once in distilledwater. They were then stained inPonceau-fuchsin solution for 5 minutes, rinsedin distilled

water and differentiated with 1% phosphotungstic acid and rinsed indistilled water. The final stain is with 1%methylbluein1% acetic acid for 15 minutes followed by several changes of distilled water, dehydration, clearing in xylene and coverslipping. As originally described by Carstairs the exactcolour of fibrin and platelets is dependent upon fixation time. With sections from tissue fixed less than 48 hours, fibrin is orange-red but is brightred in tissues fixed more than 48 hours. Since smooth muscle cells also stain red, albeit aclearly distinguishable shade, only areas with the characteristic fibrin color following Carstairs’

staining, that were also detected by immunostaining with an

anti-fibrin/fibrinogen antibody, were scored positive for fibrin

Thanks a lot, I will try this

@Andrey Dear Andrey did you try it ?

since i am also really interested to stain fibrin ? and is this method could give us a semi-quantitative indication to the fibrin deposition or not ?