The lipids are commercially available it then depends what your preferred choice to make the liposomes is (and what size liposomes you'd like). For small ones (around 25 nm diameter) you can use tip sonication if you have access to a tip sonicator. Otherwise I'd recommend getting an extruder, you can buy them here for example: http://www.avantilipids.com/index.php?option=com_content&view=article&id=509&Itemid=292&catnumber=610023 easy to use and you get liposomes of approximately the pore size you buy.

Try first with DMSO or MSM (no liposomes needed).  They are excellent carries even for peptides

As stated before, you can make the liposomes yourself. It is not rocket science, but it requires some skills. 

There plenty of lipids, of different classes, that you can use. Different lipids, different characteristics of the bilayer, different physicochemical properties of the vesicles. You can start with a very simple and cheap composition, like DPPC/Cholesterol 2:1 and then adapt it. Thin film method is the easiest and more controllable for a beginner.

Talking about methods, first you have to ask yourself if you want to encapsulate the protein directly or encapsulating it into pre-formed vesicles. Since you will use a protein, I advise you to go for the latter via freeze/thaw method. To prepare empty vesicles, the extrusion technique is monkey-proof, but the extruders are pretty costly if you have to prepare just a few samples.

Hi,

To buy ready-made liposomes, visit these pages:

http://www.lipoid.com/

* * *

Avanti Polar Lipids

https://avantilipids.com

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To make liposomes yourself in a fast and easy way, see below:

Liposome Kit: Lipid mixtures for the preparation of liposomes

lyophilized powder

http://www.sigmaaldrich.com/

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If you want to make liposomes in a safe and easy way yourself, follow these simple protocol:

1. Add the liposomal ingredients to a preheated (60°C, 5 min)

mixture of nisin (200 mg/ml) and a polyol such as glycerol

(final concentration 3%, v/v) in a heat-resistant flask such as a

pyrex beaker. Alternatively, a heat-resistant bottle with six

baffles can be used as explained and pictured in reference (49).

2. Heat the mixture at 60°C while stirring (approx. 1,000 rpm) on a

hotplate stirrer (e.g. RET basic IKAMAG1 Safety Control, IKA,

Malaysia) for a period of 45–60 min under nitrogen atmosphere.

3. For the preparation of cholesterol-containing formulations,

first dissolve the cholesterol in the aqueous phase at elevated

temperatures (c. 120°C) while stirring (approx. 1,000 rpm)

for a period of 15–30 min under nitrogen atmosphere before

adding the other phospholipid components.

4. Depending on the formulation ingredients, sample volume,

type of flask used and its number of baffles, as well as type, speed

and duration of mixing, nanometric vesicles can be produced in

one step, without the need to perform filtration or sonication.

5. Leave the nanoliposome suspension at temperatures above Tc

under an inert atmosphere such as nitrogen or argon for 1 h

to allow the sample to anneal and stabilize.

(Mozafari Method; see attached manuscript).