Hello! I am a new PhD student and unfamiliar to the mycology world, yet my current project requires a decent amount of work in it. I would like to isolate total protein from Aspergillus fumigatus and then probe it with various antibodies we have to check for binding. However, I am unfamiliar with culture and lysis techniques for fungi. I have heard of a couple freezing and grinding techniques, but haven't found many established techniques. Can anyone recommend their favorite protocol that could help me? We have also discussed looking at hyphae and mycelium, so perhaps one protocol for each would be best. Thank you!
Hello I hope you are well, I send you some links, I hope its serves you. Greetings
Article Proteomic analysis on Aspergillus strains that are useful fo...
https://www.nature.com/articles/s41598-020-63300-4
https://www.nature.com/articles/s41598-020-71297-z
Article Deletion of the Aspergillus niger Pro-Protein Processing Pro...
http://revistabionatura.com/2020.05.03.4.html
https://www.revistabionatura.com/files/2020.05.03.4.pdf
Article Characterization of Extracellular Vesicles Produced by Asper...
Dear, John,
Please have a look at the research entitled: Proteomic analysis on Aspergillus strains that are useful for industrial enzyme production , By: Takagi et al., 2020, Bioscience, Biotechnology, and Biochemistry. It may be helpful.
With regards. I attached the paper with this post.
- Badges
- Science topic
- Similar topics
- Biological Science
- Immunology
- Antibodies