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Questions related from John O'Brien
Hello! I am a new PhD student and unfamiliar to the mycology world, yet my current project requires a decent amount of work in it. I would like to isolate total protein from Aspergillus fumigatus...
21 September 2021 1,618 3 View
I am trying to isolate many plasmids for a project (ranging from 6.6Kb to 6.2Kb), and the plasmids have a pUC1 ori (high copy number from what I can tell), and Amp resistance. I am using...
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Hi everyone, I am currently isolating plasmid encoding a viral envelope protein that will be transfected into a mammalian cell line in order to make virus. After I ran a gel to monitor my plasmid...
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Hello all, I am working on a restriction digest of some DNA samples and I keep getting these dots/flame looking pattern that I can't seem to figure out. Can anyone provide insight? Thanks! Gel...
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02 February 2020 385 7 View
I am sorting through a variety of tumor cell killing assays and am wondering which one is the best/most reliable/most cost effective. I've read about chromium release assays and various forms of...
30 January 2020 2,031 1 View
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