John O'Brien

7 Questions 6 Answers 0 Followers

Questions related from John O'Brien

What is the best way to lyse and isolate proteins from Aspergillus fumigatus?

Hello! I am a new PhD student and unfamiliar to the mycology world, yet my current project requires a decent amount of work in it. I would like to isolate total protein from Aspergillus fumigatus...

21 September 2021 1,617 3 View

Why am I not getting high yield plasmid?

I am trying to isolate many plasmids for a project (ranging from 6.6Kb to 6.2Kb), and the plasmids have a pUC1 ori (high copy number from what I can tell), and Amp resistance. I am using...

10 November 2020 3,025 3 View

Does plasmid conformation matter for transfections when using a transfection reagent like Lipofectamine?

Hi everyone, I am currently isolating plasmid encoding a viral envelope protein that will be transfected into a mammalian cell line in order to make virus. After I ran a gel to monitor my plasmid...

07 October 2020 7,315 3 View

What do these dots mean on my DNA gel?

Hello all, I am working on a restriction digest of some DNA samples and I keep getting these dots/flame looking pattern that I can't seem to figure out. Can anyone provide insight? Thanks! Gel...

05 February 2020 5,718 6 View

What do these dots mean on my DNA gel?

Hello all, I am working on a restriction digest of some DNA samples and I keep getting these dots/flame looking pattern that I can't seem to figure out. Can anyone provide insight? Thanks! Gel...

02 February 2020 382 7 View

What is the best tumor cell killing assay?

I am sorting through a variety of tumor cell killing assays and am wondering which one is the best/most reliable/most cost effective. I've read about chromium release assays and various forms of...

30 January 2020 2,028 1 View

What is the best tumor cell killing assay?

I am sorting through a variety of tumor cell killing assays and am wondering which one is the best/most reliable/most cost effective. I've read about chromium release assays and various forms of...

01 January 2020 7,069 1 View