Dear Mudhir, As you mentioned my colleagues and I have used ethylene glycol in our experiment, but there are a lot of published methods for inducing renal stone in rat.

-administration the high-dose melamine/cyanuric acid [120 mg/kg/day],

http://www.ncbi.nlm.nih.gov/pubmed/20179916

-inducing renal stone by hypomagnesuric and hypocitraturic,

http://www.ncbi.nlm.nih.gov/pubmed/9280052

-inducing by tartaric acid,

http://jn.nutrition.org/content/132/12/3784.long

best

mehdi

sir i have read some research papers in urolithiasis 0.75% of ethylene glycol+ 0.5% ammonium chloride are mixed with water this method inducing renal stone

In some research papers, people also used vitamin D in high doses along with ethylene glycol 0.75% to induce nephrolithiasis.

Anything that make crystal formation with calcium, transport channels for the solute in ER or mitocondria. Most stones 70 to 80% contain mainly calcium oxalate crystals.Citrate inhibits crystal formation.

Li Y, McMartin KE. Strain differences in urinary factors that promote calcium oxalate crystal formation in the kidneys of ethylene glycol-treated rats. Am J Physiol Renal Physiol 2009; 296(5): F1080-F1087.

Dear Mudhir, it is easy to induce kidney stones in rats by adding ethylene glycol to the drinking water or by administering intraperitoneal calcitriol in low dose. I have used both methods in my investigation, using adult Wistar rats, and all the animals developed kidney calculi after two to four weeks. However, many animals also presented kidney failure and some died during treatment. More important, most animals presented a heavy burden of crystals within renal tubules. Therefore, I believe that this method does not reproduce accurately what happens in human kidney disease.

oral (ethylene glycol ) 0.75 % for a month, its easy and more abundant method used .. other chemicals were used to induce calculi are: hydroxy L-proline , Na oxalate 

How many ml of ethylene glycol you add to drinking water ? Fernando Domingos