I am planning to do global protein analysis to the oocyte of a kind of crustacean by using Mass Spec. to identify my proteins, but I am wondering about the best extraction method that I can use to get my proteins.
I have a list of NCBI protein accession numbers from different species and trying to do GO enrichment, Any tool available that may help me to do so as most work on one specie but not different...
03 April 2017 2,439 5 View
I have got set of proteins that I need to make a connection between them as they are the potential substrate to my knockdown protein. Any advice would be much appreciated.
10 November 2015 2,934 1 View
I knocked down a protein and now looking for its substrates , so need to be high confident about my proteins ID. I used the Mass-spectrometer to analyse the samples and got my protein sequences...
10 November 2015 3,949 10 View
I am trying to upload my 2-D gel images to PDQuest software virgin 8.0.1, but the software refuses them. Despite that at first it asked me to upload them in Grey scale, which I have already done,...
03 April 2014 5,891 3 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View