What is the best way to enhance adhesion of Neutrophils isolated from murine bone marrow?

19 May 2023 0 3K Report

I am after isolating neutrophils from murine bone marrow using histopaque based density gradient centrifugation. I end up with Ly6G+ neutrophils with 95% viable and 94% purity as assessed by flowcytometry. However, when I seed the cells, I see cells not getting adhered. Is there a better way to optimize the seeding so that I will better adhered cells for functional assays? Any lead is appreciated.

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