Identification of the cell line from the same patient

Please read our attached paper (Cancer Letts 331:115-121, 2013) on page 116.

A Cell ID System (Promega, Madison, WI) based on short tandem repeats (STR) was used to compare KP-N-TK cells with WBC and cells from the primary tumor of patient TK [9]. Genomic DNA was used for PCR analyses. The tool of online verification of cell line is available from DSMZ Online STR Analysis (http://www.dsmz.de/services/services-human-and-animal-cell-lines/online-str-analysis.html). The identity of two cell lines (A and B) is expressed as an evaluation value (EV) calculated as EV = (Number of coincident peaks of STR profiles between A and B) x 2 / (Total number of peaks of STR profiles in A and B). EV values greater than 0.9 indicate that the two cell types are derived from the same origin.