Need help plotting long lists of data in Graphpad Prism -?

Hi, I am generating long lists of per-cell data that I'd like to visualize as dot plots per condition. The problem is that my conditions are defined by letters rather than numbers. Or sometimes I...

11 November 2020 6,707 1 View

Elisa kit recommendations for CD3 detection from frozen spleen samples?

Hi, I have some flash-frozen mouse spleens (in the -80C) that I'd like to analyze for CD3, preferably using an Elisa kit to reduce time. I've been searching various sites, but not a lot of options...

22 October 2020 8,727 2 View

What is the best ethanol concentration for gracilaria sp?

So I use Gracilaria sp. for my research. I have tried to use 70% ethanol but I didnt get phytochem compounds. So I'm thinking to try again but I'm still confused,should i use 80% or 96%? I read...

04 June 2020 2,792 4 View

How to resolve CPCe run-time error "9"?

Hi When trying to export the analysis I have done of my images from CPCe I get the following error message which says "Run-time error "9": Subscript out of range". I have uploaded a screenshot...

30 April 2020 1,972 1 View

Is this yeast contamination in primary glial cell culture?

This is a primary glial culture (taken from pups @PD2). Picture taken at 10DIV. Astrocyte layer is completely confluent. We are actually after the microglial layer underneath the astrocytes...

03 April 2020 8,938 1 View

Protease being degraded?

I am working with a serine protease. After purification, I ran a degradation assay with casein, and it appeared to work great twice in a row (first picture representative of both attempts, first...

03 April 2020 2,582 1 View

Experience in cell culture?

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03 December 2019 1,719 6 View

Can semi-supervised machine learning be used when only a proportion of classes are defined?

Hi, I'm currently using Random Forests to classify acoustic data to corresponding bat species, however I was wondering if there is a way to also incorporate unsupervised cluster analysis into my...

13 March 2019 3,943 4 View

Which Music Composers Have Used Mathematical Formulae In Their Compositions To Represent Journeys?

I know lots of composers have created works around mathematical constructs such as the Fibonacci sequence. I would like to learn if any composers have used mathematical constructs in their music...

11 February 2019 1,153 6 View

What journals are recommended for publishing healthcare science related papers?

I am a recently qualified HCPC clinical scientist looking to start publishing papers. I specialise in critical care across paediatrics, neonates and adult ICU. I am looking for suggestions of...

02 February 2019 10,007 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

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02 March 2021 3,060 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

No title

01 March 2021 3,905 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View