Need help plotting long lists of data in Graphpad Prism -?

Hi, I am generating long lists of per-cell data that I'd like to visualize as dot plots per condition. The problem is that my conditions are defined by letters rather than numbers. Or sometimes I...

11 November 2020 6,707 1 View

Elisa kit recommendations for CD3 detection from frozen spleen samples?

Hi, I have some flash-frozen mouse spleens (in the -80C) that I'd like to analyze for CD3, preferably using an Elisa kit to reduce time. I've been searching various sites, but not a lot of options...

22 October 2020 8,727 2 View

What is the best ethanol concentration for gracilaria sp?

So I use Gracilaria sp. for my research. I have tried to use 70% ethanol but I didnt get phytochem compounds. So I'm thinking to try again but I'm still confused,should i use 80% or 96%? I read...

04 June 2020 2,792 4 View

How to resolve CPCe run-time error "9"?

Hi When trying to export the analysis I have done of my images from CPCe I get the following error message which says "Run-time error "9": Subscript out of range". I have uploaded a screenshot...

30 April 2020 1,972 1 View

Is this yeast contamination in primary glial cell culture?

This is a primary glial culture (taken from pups @PD2). Picture taken at 10DIV. Astrocyte layer is completely confluent. We are actually after the microglial layer underneath the astrocytes...

03 April 2020 8,938 1 View

Protease being degraded?

I am working with a serine protease. After purification, I ran a degradation assay with casein, and it appeared to work great twice in a row (first picture representative of both attempts, first...

03 April 2020 2,582 1 View

Experience in cell culture?

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03 December 2019 1,719 6 View

Can semi-supervised machine learning be used when only a proportion of classes are defined?

Hi, I'm currently using Random Forests to classify acoustic data to corresponding bat species, however I was wondering if there is a way to also incorporate unsupervised cluster analysis into my...

13 March 2019 3,943 4 View

Which Music Composers Have Used Mathematical Formulae In Their Compositions To Represent Journeys?

I know lots of composers have created works around mathematical constructs such as the Fibonacci sequence. I would like to learn if any composers have used mathematical constructs in their music...

11 February 2019 1,153 6 View

What journals are recommended for publishing healthcare science related papers?

I am a recently qualified HCPC clinical scientist looking to start publishing papers. I specialise in critical care across paediatrics, neonates and adult ICU. I am looking for suggestions of...

02 February 2019 10,007 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View