How can I analysis ChIP-qPCR data?

I am a beginner of Transcription factor ChIP experiment. I have taken 30ug of protein for each immunoprecipitation and the input was 1%. After immunoprecipitation, my input DNA concentration is...

01 February 2020 1,796 2 View

Which statistical test is best for additive model of genotypic study?

I am planning to do an analysis of both allelic and genotypic level of association of an particular SNP with a disease via case control study.But, I can not understand which statistical test...

07 August 2019 8,182 3 View

What would be the consequences if I take equal ratio of renilla plasmid rather than equal amount in my promoter analysis through luciferase assay?

For my promoter activity analysis, I take 0.12pmol of each different size of construct and 1:100 ratio of renilla vector. I balance the amount of total vector using a filler plasmid. 1) Construct...

02 March 2019 6,455 2 View

Why does site-directed mutagenesis not show specific band in gel?

I am doing site-directed mutagenesis using Inverse-PCR method to mutate a 10bp region of my insert. My vector plus insert size is 5.6kb. I am using Pfu polymerase from NEB to amplify my plasmid...

06 July 2018 2,164 5 View

What should be the minimum difference between your background and vector induced luciferase values?

I am doing Dual luciferase assay in a hard to transfect cell line and got these values- 1) Non-transfected- Firefly 93.16(RLU) and Renilla 123.85(RLU), so F/R is 0.75 2) Empty vector...

04 May 2018 1,981 2 View

What should be the concentration range of my drug to check its activity in cell lines?

I want to check the activity and the efficacy of 5 different drugs in cell lines to evaluate the best one based on their potency and reduced toxic effects. In many papers, researches did take the...

10 November 2017 8,445 11 View

What is Biological replicate in cell culture experiments?

I am a beginner of cell culture experiments and just have started some experiments with Hela cell line.In research papers, it is always highlighted that, each experiment has been done in...

07 August 2017 6,930 2 View

What is true Biological replicate in cell culture experiments?

In my cell culture experiments, first I have revived a vial of cells, subcultured it, then extracted RNA from a particular no of cells for my experiments.After RNA isolation, I have freezed my...

06 July 2017 8,334 1 View

Why did endogeneous controls not show any band in western blot?

I am a beginner of western blot and my target protein if 124kDA whereas the endogeneous controls are 41kDA and 55kDA. I did wet transfer at 100V, 80mins and successfully got the band for target...

05 June 2017 4,613 5 View

Why do the low molecular weight proteins not show any band in western blot?

I am a beginner of western blot and my target protein if 124kDA whereas the endogeneous controls are 41kDA and 55kDA. I did wet transfer at 100V, 80mins and successfully got the band for target...

04 May 2017 9,128 10 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View