What is the best strategy to produce triploid plants of the trees with long maturity period?

More Saba Saboori's questions See All

What is the best way to calculate the weight of each lipid component needed to achieve the desired molar ratios?

I'm currently working on preparing a 10 mL liposome solution containing HSPC, mPEG2000-DSPE, and cholesterol at a molar ratio of 55/40/5. can anybody advise on the best way to calculate the weight...

26 June 2024 1,135 0 View

Τ-Random Acceleration Molecular Dynamics (τ-RAMD) Simulation?

I want to calculate τ-Random Acceleration Molecular Dynamics (τ-RAMD) Simulation using NAMD/VMD software? Kindly provide input parameters that are essential to run these simulation. I will be very...

31 May 2024 2,662 2 View

How to maintain structural integrity during fixation of termite worker?

I am working on termites and doing in situ hybridization but at the end, I can't see cells in specimen slides. my question is how to maintain the structural integrity during fixation or whole...

18 April 2024 279 0 View

What should be the minimum time interval required for longitudinal studies to be conducted?

can this time interval be for 6 months ? if the data is collected twice in a period of year. is this considered longitudinal studies ? what is the degree of freedom if some of the participants...

07 March 2024 1,111 4 View

If you wanted to separate the cell membrane, which hypotonic solution would you choose to lyse the cancer cell line?

1- Sucrose 10% containing protease inhibitors 2-10 mm Tris-HCL, pH = 7.5 and supplemented with the ready-to-use 1× protease inhibitor cocktail 3-Tris buffer (pH = 7.4) containing 10 mM Tris, 10...

22 February 2024 9,698 0 View

HIV high prevalence in Pakistan. Reasons and under treatment patients ?

HIV uncontrolled in south Punjab and Sindh

04 February 2024 1,891 2 View

Is it possible to solve partial differential equations with AI and machine learning? If yes what is a best source for this topic?

As we know, in partial differential equations, we deal with pure mathematics or computational methods, so in what aspects, machine learning can help us to solve partial differential...

15 January 2024 1,951 2 View

What are the possible reasons behind a high polydispersity index (PDI) in polyglycerol sebacate (PGS) prepolymer?

Hi everyone, I'd be thankful if anyone having experience in polyglycerol sebacate synthesis could tell me what parameters could lead to a high PDI? thanks

15 December 2023 2,685 1 View

What is the difference between Double Network (DN)-based hydrogels and Interpenetrating Polymer Network (IPN)-based hydrogels?

Hello everyone, I wanted to know how do these two networks differ from one another? Are DNs a subgroup of IPNS, or are they completely different? Thank you in advance.

06 December 2023 9,859 2 View

What is methodology of abdul rehman bin ali bin juizi in his book" فنون الافنان عجائب علوم القرآن"?

i need biography of the author plus need methodology of his book in arabic language..

25 November 2023 3,555 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?

I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...

03 August 2024 9,918 0 View