Saba Saboori

11 Questions 11 Answers 0 Followers

Questions related from Saba Saboori

Any suggestion of a book or scientific text for step by step breeding program for flower bulb plants?

Read 3 answers by scientists to the question asked by Saba Saboori on Oct 29, 2014

29 October 2014 8,299 2 View

How can I measure the callus size without explant corruption or without weighing callus in vitro?

Is there any way in this regards?

30 August 2014 1,745 15 View

Does anybody have experience of working with plants which generate substantial roots during all stages of micropropagation?

I am looking for the ways to control this phenomenon as well as some references related to it.

07 August 2014 2,901 4 View

How can I control the hyperhydricity in the micropropagation of plants?

Would you please introduce some good papers or useful techniques in controlling hyperhyricity?

08 June 2014 606 15 View

How can I inhibit continuous rooting in plant micropropagation?

2 weeks after culture of apical bud in proliferation media (MS medium) with BA or other cytokines in vitro, it starts huge amounts of rooting till the end of the process.

08 June 2014 2,194 5 View

Can someone advise on how to get bands with chloroplastic genes for phylogenetic analysis of euphorbia species?

the PCR program is as follow: 94 C 5min, 35 cycles [(94 C 50 ") (58 C 50") (72 C 1min)] (72 C 7min) (trnH forward and psbA reverse primers)

15 January 2014 935 1 View

How to evaluate the proliferation rate of in vitro cultured plants with compact growth habit and small leaves?

It is difficult to count the number of proliferated shoots generating from the apical bud (as the source explant). What criteria I can consider for evaluating the proliferation rate of this...

27 October 2013 5,712 19 View

Can anybody introduce an efficient DNA extraction method for woody plants that have high percentages of phenol and polysacharid?

I want to extract DNA of some plants such as pomegranate, I am looking for an easy and efficient method, Can anybody help?

30 June 2013 9,313 30 View

Any suggestions on why my explants in vitro did not produce any root in rooting medium after a month?

I used different concentrations of IBA and NAA less than 1mg/l) or even ms medium without any growth regulators for rooting the explants but it didn't work after one month. Does anyone have any idea?

02 June 2013 2,064 23 View

How long does an experiment need to be in order to decide the best disinfection method for plants in vitro culture?

I am evaluating the best disinfection method for in vitro culture of a wild plant. What is the minimum required time for the experiment? Is one month enough?

19 May 2013 4,927 10 View

Is there any easier method other than silver staining for staining DNA fragments in polyacrilamide gel?

I want to stain DNA fragments in polyacrilamide gel but it takes too long to do so. Is there any easier protocol for this experiment?

18 May 2013 575 4 View