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Dear fellow scientists!!! I have been doing cloning lately and have encountered some problems during my experiments, for example, I can get none or very few colonies after transformation into...
03 December 2020 2,365 11 View
Hello Everyone, I have some questions related to a drug called Cobalt 2 chloride hexahydrate. I am currently preparing an experiment related to Hypoxia. I bought a drug called " Cobalt 2...
11 May 2020 4,967 4 View
Hello Everyone, I have some questions related to cell plating in 6-well plate. I have plated my cells (miaPaca-2 and PANc-1 cell lines) in 6-well plates. The Media I use is DMEM (with 10% FBS...
09 March 2020 8,419 3 View
Hello, Everyone. I have some questions related to western blot as I am new to western blot. I tried to detect the LC3B, however I got diffused bands as I have attached in the file (Please find the...
13 November 2019 8,404 13 View
Good morning, Everyone! I have some questions related to Real-time PCR. I have checked the expression of a microRNA (microRNA primer) in monolayer and spheroid cells. The first time I run the...
14 June 2019 4,466 12 View
I am looking for a standard protocol of how to design a good primer for RT-PCR. Could anyone recommend me the standard protocol of the design of (microRNA or gene) primers for RT-PCR as a reference?
14 June 2019 9,477 5 View
Firstly, I have extracted RNA samples by using QIAzol, and then I also tried to use TRIzol to extract RNA samples. Can anyone tell me which RNA extraction method is better between the extraction...
29 November 2018 8,953 4 View
