What is the best solvent for extraction of PAHs from soil/sediment sample?

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Need a reference on pig residual analysis report and the parameters containing in it, for an oil and gas pipeline industry. Has anybody done it?

Pigging is basically a cleansing process followed in all pipeline industries after the product has been delivered. There are many types of pigs that are inserted inside the pipeline for this...

27 June 2024 1,221 1 View

HI, How to find phase response in CST?

I'm simulating a metamaterial unit cell. I want to plot Frequency Vs Phase Response. Also I want to extract permeability and permittivity. Kindly share the steps.

23 June 2024 3,446 1 View

Yeast Cell Glucose Uptake Assay ? Can yeast release more glucose into the reaction, when treated with test compound ?

Hi all, We performed Yeast-Cell-Glucose-Uptake assay to estimate the antidiabetic potential of the test compound. The Metformin treated yeast cells, resulted in taking up more glucse than normal...

02 April 2024 7,657 2 View

Suggest good geospatial data security papers ?

pursuing phd on geospatial data security.suggest good paper on robust invisible blind watermarking for copyright protection

12 March 2024 4,917 2 View

What is the difference between the single microgrid and multi microgrid? why we consider Multi microgrid in an area but not as a single microgrid ?

in a community area, there is an multi microgrid systems, why we call it as multi microgrid system but not treated as single microgrid system. and difference between single microgrid and multi...

03 March 2024 9,306 1 View

Wire bonding issue (Au/Ti/GaN). Any feasible solutions please?

I am facing an issue of wire bonding, trying to ball bond Au wire onto a metal stack/semiconductor (Au/Ti/GaN). Thickness of Au = 200 nm, Thickness of Ti = 20 nm. I observe that the metal...

07 January 2024 4,813 1 View

How to calculate the precision for a RT PCR assay?

I am studying precision for my assay/kit with the following parameters followed: Panel members to be tested: 1. Weak positive 2. Medium positive 3. Strong positive 4. Negative All these were...

11 December 2023 1,812 1 View

How to add my articles in research gate?

how can i add all my articles and journals in research gate including those published in research gate?

22 November 2023 5,926 3 View

Statins causes liver damage upon prolonged use. To control it, given natural with hepatoprotective activity. But results HDI! what might be the cause?

Atorvastatin used to treat Atherosclerosis, seems to causes hepatoxicity upon prolonged usage. So, to inhibit such problem we have introduced an natural substances which is reported for the...

09 November 2023 587 0 View

Why monocytes adhere while differentiating them as Dendritic cells?

I prepared PBMCs,s from human blood and differentiated them as immature dendritic cells in this process, monocytes adhere to the plates. What could be the reason for this phenotype?

09 November 2023 8,266 2 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

Can i use the protease inhibitors during cell membrane vesicle preparation ？?

I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...

30 July 2024 7,077 1 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

How should we increase the quality of RNA extraction?

I’m having difficulty achieving high RNA integrity in my samples. Although the 260/280 and 260/230 ratios are satisfactory after RNA extraction, the RNA samples show signs of degradation when...

22 July 2024 155 4 View

The best source for amplification of ADAM17 prodomain?

hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...

21 July 2024 8,660 1 View

Is there a method that I can measure alcohol dehydrogenase activity using NAD+ in enzyme extraction from plants?

17 July 2024 935 1 View