Is it strictly recommended to run samples in duplicate during SNP genotyping analysis?

02 February 2021 8,877 3 View

How to present and discuss results, when methodology is not data-based?

Dear Everyone! I'd ask you opinion about a problem, which is returning in my "softer" research field. Time to time I work on research projects, which do not have available data and/or the data...

29 December 2020 7,637 6 View

How would you describe the most relevant research questions and directions in urban air pollution, if I'm specially interested in transportation?

I am relatively new to this field, my research interests are smart cities, shared mobility, transport service integration. We have data about CO, NO2, O3, PM10 from several cities. I would like to...

11 November 2020 9,099 4 View

What are the best scales to measure the role of Artificial Intelligence in trade/commerce?

I'm looking for scales to measure the role of Artificial Intelligence in trade/commerce. I'd be glad if you could recommend some valuable papers in this field too. Thanks a lot!

24 June 2020 4,209 3 View

Golgi-cox troubleshooting?

Hello there, I am having mixed results with golgi-cox staining and I was wondering if anyone has experience/insight into the individual stepwise process. My issue is that the neurons are not...

03 November 2019 2,911 2 View

Can I store my cells for few days at 4 C before FACs analysis?

I want to ask if it is possible to store my cells 24 hours post transfection at 4 C for 4-5 days (Non-fixed) just in normal media as one of my conjugated antibody shipment is delayed and will be...

10 July 2019 5,109 4 View

How to determine molecular weight of disordered peptides from diffusion NMR data?

From diffusion NMR (DOSY) experiments, diffusion coefficients are available for ~40 AA disordered peptides. Is there a method to calculate the molecular weights using these data, that is suitable...

08 July 2019 703 3 View

What is the role of the artificial intelligence (AI) in generating social innovations?

Do you know any papers discussing the the role of AI in social innovations? I'm also interested in the best practices in this field. I intend to write a literature review. Thanks for your...

01 March 2019 2,105 3 View

Are there any data available about the mechanism of loss of effectivity to immune checkpoint inhibitors?

Despite the good effectivity of the immune checkpoint inhibitors a proportion of patients will face relapse, and will be resistant to this treatment. I am intereseted if we know anything about...

03 January 2019 8,134 3 View

Best sources for analyzing Social Innovation?

What are the best sources for analyzing social innovation? I mean journal articles, papers, research reports, etc, the "must reads". Thanks for your help!

18 October 2018 8,798 1 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

How to improve RNA isolation?

Hi, I have been struggling with a weird issue for my RNA samples. I used the Qiagen RNA plus mini kit. For some reason no matter how careful I am with the washes, when I measure the RNA, I get...

22 February 2021 1,286 6 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

How to control the variants vof covid 19 in second and third waves?

A the world find means to control covid 19 by vaccination ,new variants came to spread and make the world in fear and danger, to understand the conduct of these variants, es constantly change...

21 February 2021 3,080 6 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

FDC and FRC cell populations in mouse spleen?

I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...

17 February 2021 3,506 4 View