How to calculate and interpret bacterial mutation frequency?

In my experiment I have checked the bacterial mutation frequency after different treatment. The treated bacteria were spread in a plate containing an antimicrobial. The mutation frequency was...

11 December 2018 5,701 4 View

What are the references for antibacterial exposure study using agar dilution method?

I want to perform an experiment for evaluation of the effect of antimicrobial exposure on antibiotic sensitivity of fish pathogenic bacteria. I want to go for agar dilution for exposing the...

03 April 2018 5,090 1 View

What are the steps for analysis of a set of length-weight data for different condition factors of fish using Microsoft Excel or any other software?

Please provide details steps for analysis of a set of length-weight data for calculating the condition factor. How the set of data can be converted into a single value of condition factor. What...

02 March 2018 1,435 0 View

Kluyvera intermedia is fish pathogenic or not?

Kindly provide link of such report with Kluyvera intermedia infection in fish? if any

08 September 2017 2,407 3 View

Isolation procedure of Polyethylene degrading bacteria?

Please suggest some standard pocedurte for isolation of polyethylene degrading bacteria from soil and water sample. Standard media and polythene sample is varying in different articles besides the...

31 December 2016 2,737 3 View

What are the standard protocol for Bioaccumulation, Bioconcentration and Biomagnification study in Fish?

07 August 2016 1,629 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

How to compare different concentrations of nanoparticles for characterization from ICP-MS?

I took measurements of different concentrations of 15 nm and 5 nm Au nano particle solutions. I'm trying to determine which concentration I measured gives the best reading overall. I also...

02 March 2021 7,626 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View