What is the best process for estimating Triclosan from sediment samples?

What is the best and easiest protocol for extracting Triclosan from sediment and further estimation using HPLC, GC-MS or LC-MS? Please suggest some easy and dependable protocol.

02 March 2019 8,473 5 View

How to calculate and interpret bacterial mutation frequency?

In my experiment I have checked the bacterial mutation frequency after different treatment. The treated bacteria were spread in a plate containing an antimicrobial. The mutation frequency was...

11 December 2018 5,701 4 View

What are the references for antibacterial exposure study using agar dilution method?

I want to perform an experiment for evaluation of the effect of antimicrobial exposure on antibiotic sensitivity of fish pathogenic bacteria. I want to go for agar dilution for exposing the...

03 April 2018 5,090 1 View

What are the steps for analysis of a set of length-weight data for different condition factors of fish using Microsoft Excel or any other software?

Please provide details steps for analysis of a set of length-weight data for calculating the condition factor. How the set of data can be converted into a single value of condition factor. What...

02 March 2018 1,435 0 View

Isolation procedure of Polyethylene degrading bacteria?

Please suggest some standard pocedurte for isolation of polyethylene degrading bacteria from soil and water sample. Standard media and polythene sample is varying in different articles besides the...

31 December 2016 2,737 3 View

What are the standard protocol for Bioaccumulation, Bioconcentration and Biomagnification study in Fish?

07 August 2016 1,629 3 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Hi i want to ask that in order to find out LC 50 (96 hours) of particular toxin for fish. Should we keep the animals with food or without food?

if we expose the fish after acclimatization to particular toxin in order to find out LC 50 (96 hours).Should we keep the fish off feed or with feed for that duration. Some literature show that...

24 February 2021 357 2 View

Measuring Total immunoglobulin whole-body of fish larvae?

Hi Dear All, I prepared the extract of fish larvae whole body with PBS. Then protein concentration of the whole-body extraction samples determined by the Folin-Ceiocalteu-Phenol method (Lowry...

17 February 2021 1,971 3 View

What is the identity of additional bands found during fish gut microbiome 16S V4 PCR amplification?

Hi, I am carrying out test PCRs for 16S amplicon sequencing on Illumina MiSeq. I am using standard V4 primers: 515F/806R. The aim is to profile the microbiome of fish guts under different...

16 February 2021 5,668 6 View

Is this the fragment of a sauropod tooth?

Dear colleagues. Fragments of this species are very rare from a terrestrial Lower Cretaceous locality from Germany. Up to now from this locality sauropods could not be proved with certainty. Other...

14 February 2021 2,310 1 View

Problem to dissolve 50%wt Glycerol in 4%wt PVOH 28-99 ??

Hello everyone, I am trying to prepare a solution out of PVA(Polyvinyl alcohol)_Glycerol_Wasser . (4% PVA 28-99 and 50% Glycerin) To prepare this solution I first add PVA to 80degree water and...

06 February 2021 2,300 3 View

How to remove inter-year variation from my assessment of seasonal variation?

I have a 30 year dataset of fish sampling. I know from previous analyses that there is a huge amount of variation between years in catch rates. What I would like to do is create a plot that shows...

01 February 2021 3,980 5 View

How can we measure disease severity of a pathogen in a crop?

severity index, measuring method and procedure detailed

01 February 2021 1,160 11 View