What is the best phenotypic screening assay for Glioblastoma cells ?

23 April 2014 1 5K Report

I need to do a phenotypic assay for several compounds to see their effects on cytotoxicity, apoptosis, morphology, and cell analysis assay on Glioblastoma cells.

Robert Kiss

Dear Issa,

I do not know whether an assay could be "the best".

An essay remains an essay ...

We are using computer-assisted phase-contrast microscopy as illustrated in the three films here attached (this is a GBM cell line; Ct = control; CS = cardiotonic steroid [cytotoxic]; Narc = narciclasine [cytostatic]). You can see that many phenotypic characteristics can be observed with respect to these two compounds when compared to the control, or between themselves.

I am also arraching here two articles to show you how we can take benefit of this approach.

Best regards

Robert

Badges
Science topic

Similar topics
Microbiology
Assays

More Issa Amara's questions See All

What is suitable temperature at reaction mixture of lipase activity assay?

I use para nitrophenyl palmitate substrate with tris buffer ph 8... i read many temperature for Yellow color of para nitro phenol production.. 37 and 50 and 40 at water bath ... I confused as i...

04 February 2021 9,100 5 View

What is the relationship that determines the depth of the mechanical cultivator with the age or size of the weed For crops planted in lines?

the great depth of the cultivator may lead to passage under the roots without killing them and may give negative results to break up the soil below the roots, increasing their activity, especially...

03 February 2021 3,198 7 View

Does the subsoil have an effect on the spread of weeds whose roots are more than 35 cm deep?

For weeds whose roots deepen more than the level of subsoil, they may be affected by this layer, for example, determined by height and growth, as well as limited to flower production and seeds.

01 February 2021 1,940 4 View

Can I measure serum and not tissue succinate dehydrogenase?

Dear Colleagues I am interested in evaluating the concentration of SERUM succinate dehydrogenase in a certain type of cancer. I found an ELISA kit for it however, I can barely find any papers...

16 October 2020 547 1 View

How do you develop a budget in a nursing research?

Hello, I am developing an online education program teaching about COVID-19. The method of teaching will be that participants will use their own phone where they have to install WhatsApp (free of...

28 September 2020 5,522 4 View

Which unsupervised learning methods can be used to detect fake users?

In an online website, some users may create multiple fake users to promote (like/comment) on their own comments/posts. For example, in Instagram to make their comment be seen at the top of the...

12 August 2020 3,736 12 View

My melanoma cell line doesn't grow well suddenly, May i know the possible reasons for that?

I have 92-1 of melanoma cell line , when i thawed them they were totally normal , but after first passage , the cells begun not growing well and not totally attaching on the bottom of the flask...

30 June 2020 3,622 4 View

Which operators that are not linear combination of orthogonal projections ?

If B(H) is the algebra of bounded linear operators acting on an infinite dimensional complex Hilbert space, then which elements of B(H) that can't be written as a linear combination of orthogonal...

22 March 2020 9,704 13 View

How to compare pre-trained models with traditional models in the field of NLP?

There are new models such as BERT that requires pre-training and fine-tuning. And there are the traditional models, such as DecisionTree and SVM, which requires us to extract features from the...

20 March 2020 7,784 2 View

Possible reason to change the color of carbon dots from greenish into blue after immobilization process?

Dear experts, Liquid carbon dots (CDs ) were synthesized with greenish color under UV-light. However, after mixing with PVA polymer, the color turned to blue with enhanced quantum yield (QY). what...

16 March 2020 5,304 4 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View