How do we remove carbon, copier, and another mineral from the Biosynthesis of Zinc oxide nanoparticles?
(I have mixed the supernatant of bacteria with (20 mM ) of zinc sulfate for preparation of the zinc oxide nanoparticles). Then, I washed the precipitate with PBS and D.W three times.
After that, I got the particle size by DLS and TEM, but the result of EDX is a carbon (C) 97%.
Hope to see your answer with a reference or without reference.
Regards,
Ahmed
Dear Dr. Gorshkov
Thank you so much for your reply. sometimes I got 2% of zinc by EDX. I can't consider it as Carbon because I need to get pure zinc. So if you have any knowledge about how I can remove the carbon and get the pure nanoparticles.
Do you think the glucose in the culture media is responsible for Carbon formation around the nanoparticles or covers it? Bacteria use glucose as a carbon source during the growing.
regards,
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